14. Mass Spectrometry for Steroid Analysis

  1. Mike S. Lee
  1. William J. Griffiths,
  2. Michael Ogundare,
  3. Anna Meljon and
  4. Yuqin Wang

Published Online: 21 MAY 2012

DOI: 10.1002/9781118180730.ch14

Mass Spectrometry Handbook

Mass Spectrometry Handbook

How to Cite

Griffiths, W. J., Ogundare, M., Meljon, A. and Wang, Y. (2012) Mass Spectrometry for Steroid Analysis, in Mass Spectrometry Handbook (ed M. S. Lee), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118180730.ch14

Editor Information

  1. Milestone Development Services, USA

Author Information

  1. Institute of Mass Spectrometry, School of Medicine, Swansea University, Swansea, Wales, UK

Publication History

  1. Published Online: 21 MAY 2012
  2. Published Print: 18 APR 2012

Book Series:

  1. Wiley Series on Pharmaceutical Science and Biotechnology: Practices, Applications and Methods

Book Series Editors:

  1. Mike S. Lee

Series Editor Information

  1. Milestone Development Services, USA

ISBN Information

Print ISBN: 9780470536735

Online ISBN: 9781118180730



  • mass spectrometry for steroid analysis;
  • steroids and new properties, MS for unbiased, quantitative analysis;
  • steroids by direct infusion ESI-MS, and ESI-MS/MS in steroid analysis;
  • FAB–MS for bile acids and steroid conjugate analysis;
  • new applications in secosterol analysis


This chapter contains sections titled:

  • Introduction

  • Sample Preparation

  • Gas Chromatography–Mass Spectrometry

  • ESI-MS and Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)

  • Application of MS to Steroid Analysis

  • New Applications

  • Protocols

  • Conclusions

  • Acknowledgment

  • References

  • Appendix 14.1

  • Appendix 14.2

  • Appendix 14.3

  • Appendix 14.4