2. Mass Spectrometry-Based Methods to Investigate Posttranslational Protein Modifications by Lipid Peroxidation Products

  1. Mike S. Lee
  1. Navin Rauniyar and
  2. Laszlo Prokai

Published Online: 21 MAY 2012

DOI: 10.1002/9781118180730.ch2

Mass Spectrometry Handbook

Mass Spectrometry Handbook

How to Cite

Rauniyar, N. and Prokai, L. (2012) Mass Spectrometry-Based Methods to Investigate Posttranslational Protein Modifications by Lipid Peroxidation Products, in Mass Spectrometry Handbook (ed M. S. Lee), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118180730.ch2

Editor Information

  1. Milestone Development Services, USA

Author Information

  1. Department of Molecular Biology & Immunology, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX, USA

Publication History

  1. Published Online: 21 MAY 2012
  2. Published Print: 18 APR 2012

Book Series:

  1. Wiley Series on Pharmaceutical Science and Biotechnology: Practices, Applications and Methods

Book Series Editors:

  1. Mike S. Lee

Series Editor Information

  1. Milestone Development Services, USA

ISBN Information

Print ISBN: 9780470536735

Online ISBN: 9781118180730

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Keywords:

  • MS for protein identification and PTM characterization;
  • protein covalent modification, posttranslational steps;
  • modifications, rendering protein functionality in cells;
  • PTM identification by HNE, revealing protein targets;
  • SPH with CID, ECD, in HNE-induced carbonylation via MA

Summary

This chapter contains sections titled:

  • Procedure Control

  • Identification of HNE-Modified Peptides in Biological Samples by Solid-Phase Enrichment and Nano-LC–ESI-MS/MS

  • Reagents and Standards

  • Commentary

  • Acknowledgments

  • References