4. Methodologies for Identifying Microorganisms and Viruses by Mass Cataloging of RNAs

  1. Mike S. Lee
  1. George W. Jackson1,
  2. Rafal Drabek1,
  3. Mithil Soni1,
  4. Roger McNichols1,
  5. Richard C. Willson2,3 and
  6. George E. Fox4

Published Online: 21 MAY 2012

DOI: 10.1002/9781118180730.ch4

Mass Spectrometry Handbook

Mass Spectrometry Handbook

How to Cite

Jackson, G. W., Drabek, R., Soni, M., McNichols, R., Willson, R. C. and Fox, G. E. (2012) Methodologies for Identifying Microorganisms and Viruses by Mass Cataloging of RNAs, in Mass Spectrometry Handbook (ed M. S. Lee), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118180730.ch4

Editor Information

  1. Milestone Development Services, USA

Author Information

  1. 1

    BioTex, Inc, Houston, TX, USA

  2. 2

    Department of Chemical and Biomolecular Engineering, Department of Biology and Biochemistry, University of Houston, TX, USA

  3. 3

    The Methodist Hospital Research Institute, Houston, TX, USA

  4. 4

    Department of Biology and Biochemistry, University of Houston, Texas, USA

Publication History

  1. Published Online: 21 MAY 2012
  2. Published Print: 18 APR 2012

Book Series:

  1. Wiley Series on Pharmaceutical Science and Biotechnology: Practices, Applications and Methods

Book Series Editors:

  1. Mike S. Lee

Series Editor Information

  1. Milestone Development Services, USA

ISBN Information

Print ISBN: 9780470536735

Online ISBN: 9781118180730

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Keywords:

  • microorganisms and virus identification, RNA mass cataloging;
  • microbial genotyping, and rapid molecular identification;
  • mass cataloging of bacterial 16S RNA, and database construction;
  • universal primers directed at bacterial 16S rRNA;
  • methodologies, PCR, transcription, and enzymatic fragmentation

Summary

This chapter contains sections titled:

  • Introduction: The Importance of Microbial Genotyping

  • Informatics: Enabling Aspects of 16S R RNA and Database Construction

  • Experimental Methods: PCR, Transcription, and Enzymatic Fragmentation

  • Database Comparison and Results: Typical Results of Bacterial and Viral Genotyping

  • Discussion

  • Acknowledgments

  • References