14. Detection of Antibodies to Poly(Ethylene Glycol) Polymers Using Double-Antigen-Bridging Immunogenicity ELISA

  1. Yashwant Pathak2 and
  2. Simon Benita3
  1. Yijuan Liu,
  2. Helen Reidler,
  3. Jing Pan,
  4. David Milunic,
  5. Dujie Qin,
  6. Dave Chen,
  7. Yli Remo Vallejo and
  8. Ray Yin

Published Online: 23 APR 2012

DOI: 10.1002/9781118229019.ch14

Antibody-Mediated Drug Delivery Systems: Concepts, Technology, and Applications

Antibody-Mediated Drug Delivery Systems: Concepts, Technology, and Applications

How to Cite

Liu, Y., Reidler, H., Pan, J., Milunic, D., Qin, D., Chen, D., Vallejo, Y. R. and Yin, R. (2012) Detection of Antibodies to Poly(Ethylene Glycol) Polymers Using Double-Antigen-Bridging Immunogenicity ELISA, in Antibody-Mediated Drug Delivery Systems: Concepts, Technology, and Applications (eds Y. Pathak and S. Benita), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118229019.ch14

Editor Information

  1. 2

    Department of Pharmaceutical Sciences, College of Pharmacy, University of South Florida, Tampa, Florida, USA

  2. 3

    The Institute for Drug Research of the School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel

Author Information

  1. ANP Technologies, Inc., Newark, Delaware, USA

Publication History

  1. Published Online: 23 APR 2012
  2. Published Print: 11 MAY 2012

ISBN Information

Print ISBN: 9780470612811

Online ISBN: 9781118229019

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Keywords:

  • double-antigen-bridging, ELISA, detection of anti-PEG antibodies;
  • immunogenicity assays and ADA detection in biotherapeutics;
  • antibody specificity in positive human serum samples;
  • PEG-specific, of PEGylated biotherapeutics, drug efficacy, safety;
  • dose–response curves and assay reproducibility

Summary

This chapter contains sections titled:

  • Introduction

  • Methods

  • Results

  • Discussion

  • References