7. Permeability—PAMPA

  1. Alex Avdeef1,2

Published Online: 7 MAY 2012

DOI: 10.1002/9781118286067.ch7

Absorption and Drug Development: Solubility, Permeability, and Charge State, Second Edition

Absorption and Drug Development: Solubility, Permeability, and Charge State, Second Edition

How to Cite

Avdeef, A. (2012) Permeability—PAMPA, in Absorption and Drug Development: Solubility, Permeability, and Charge State, Second Edition, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118286067.ch7

Author Information

  1. 1

    Cambridge, Massachusetts, USA

  2. 2

    Boston, Massachusetts, USA

Publication History

  1. Published Online: 7 MAY 2012
  2. Published Print: 11 MAY 2012

ISBN Information

Print ISBN: 9781118057452

Online ISBN: 9781118286067

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Keywords:

  • permeability—PAMPA model;
  • permeability measurement, predicting HJP and HIA;
  • biological membranes, and lipid compositions;
  • Iso-pH equations with membrane retention;
  • PAMPA paramembrane water channels

Summary

This chapter contains sections titled:

  • Permeability in the Gastrointestinal Tract

  • Historical Developments in Permeability Models

  • Rise of PAMPA—A Useful Tool in Early Drug Discovery

  • PAMPA-HDM, -DOPC, -DS Models Compared

  • Modeling Biological Membranes

  • Permeability–pH Relationship and the Mitigating Effect of the Aqueous Boundary Layer

  • pKaFLUX-Optimized Design (pOD)

  • Cosolvent PAMPA

  • UV versus LC/MS Detection

  • Assay Time Points

  • Buffer Effects

  • Apparent Filter Porosity

  • PAMPA Errors: Intra-Plate and Inter-Plate Reproducibility

  • Human Intestinal Absorption (HIA) and PAMPA

  • Permeation of Permanently Charged Molecules

  • Permeation of Zwitterions/Ampholytes—In Combo PAMPA

  • PAMPA in Formulation: Solubilizing Excipient Effects

  • Database of Double-Sink PAMPA log P0, log Pm6.5, and log Pm7.4

  • Quick Start: Double-Sink PAMPA of Metoprolol

  • Permeability Equations

  • PAMPA Paramembrane Water Channels

  • References