11. Phage Libraries

  1. W. John W. Morrow PhD, DSc, FRCPath2,
  2. Nadeem A. Sheikh PhD3,
  3. Clint S. Schmidt PhD4 and
  4. D. Huw Davies PhD5
  1. Aaron K. Sato PhD

Published Online: 20 JUN 2012

DOI: 10.1002/9781118345313.ch11

Vaccinology: Principles and Practice

Vaccinology: Principles and Practice

How to Cite

Sato, A. K. (2012) Phage Libraries, in Vaccinology: Principles and Practice (eds W. J. W. Morrow, N. A. Sheikh, C. S. Schmidt and D. H. Davies), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781118345313.ch11

Editor Information

  1. 2

    Seattle, WA, USA

  2. 3

    Dendreon Corporation, Seattle, WA, USA

  3. 4

    NovaDigm Therapeutics, Inc., Grand Forks, ND, USA

  4. 5

    University of California at Irvine, Irvine, CA, USA

Author Information

  1. OncoMed Pharmaceuticals, Redwood City, CA, USA

Publication History

  1. Published Online: 20 JUN 2012
  2. Published Print: 3 AUG 2012

ISBN Information

Print ISBN: 9781405185745

Online ISBN: 9781118345313

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Keywords:

  • phage;
  • display;
  • library;
  • peptide;
  • antibody;
  • mimotope;
  • vaccine;
  • epitope;
  • vaccine;
  • immune;
  • antigen;
  • mapping

Summary

Phage display is a technology that has been applied to a wide array of scientific fields, such as therapeutics, diagnostics, imaging agents, affinity media, and vaccine development. In this latter field, this chapter describes how phage display libraries are used as tools to dissect the immune response against foreign pathogens and diseases. Using peptide phage display (PPD), many authors have discovered novel peptides that serve as surrogates for antigens. By selecting PPD libraries against antibodies directed to key epitopes on antigens, small peptides are discovered that bind the antigen-binding site on the selected antibody. When used to immunize animals, these peptides (mimotopes) can mimic the antigen and elicit an immune response against the parental antigen. Besides using synthetic peptides for immunization, many groups have immunized animals directly with the selected peptide(s) on phage and found these vaccines yield a more robust immune response than the peptide alone. In cases where the antigen is not known, many laboratories have discovered the targeted antigens in an immune response by selecting high titer polyclonal sera on phage display libraries (peptide and cDNA). Once the antigen is discovered, PPD is often used to further epitope map the antigen-antibody interaction and also to develop mimotopes to be used as vaccines. This chapter provides examples of each of these applications and highlights how PPD libraries are used for novel vaccine strategies.