20. Archival Toxicoepigenetics: Molecular Analysis of Modified DNA from Preserved Tissues in Toxicology Studies

  1. Saura C. Sahu
  1. B. Alex Merrick

Published Online: 8 AUG 2012

DOI: 10.1002/9781118349045.ch20

Toxicology and Epigenetics

Toxicology and Epigenetics

How to Cite

Merrick, B. A. (2012) Archival Toxicoepigenetics: Molecular Analysis of Modified DNA from Preserved Tissues in Toxicology Studies, in Toxicology and Epigenetics (ed S. C. Sahu), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781118349045.ch20

Editor Information

  1. Division of Toxicology, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, Maryland, USA

Author Information

  1. Molecular Toxicology and Informatics Group, Biomolecular Screening Branch, National Toxicology Program Division, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 7 SEP 2012

ISBN Information

Print ISBN: 9781119976097

Online ISBN: 9781118349045

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Keywords:

  • archival toxicoepigenetics, of modified DNA from preserved tissues;
  • biobanking or ‘the storage of donor-traceable biological materials’;
  • DNA, RNA, micro-RNA, from paraffinized tissues, ‘breathing new life’;
  • tissue preservation on protein/nucleic, epigenetics in preserved archival;
  • aqueous formaldehyde forms, protein reaction in tissue preservation;
  • nucleic acid fate, declining in time spent in formaldehyde, fixation affect;
  • nucleic acids from fixed/embedded, DNA for epigenetics;
  • preserved toxicology, epigenetics in malignancies/toxicity phenotypes

Summary

This chapter contains sections titled:

  • Introduction

  • Preservation of tissue: effects on protein and nucleic acids

  • Extraction of nucleic acids from fixed or embedded tissues

  • Analysis of methylated DNA for epigenetics

  • Survey of epigenetic studies using formalin preserved tissues

  • Prospects for toxicoepigenetics in preserved tissues

  • Conclusion

  • References