22. Methods of Global Epigenomic Profiling

  1. Saura C. Sahu
  1. Michael W. Y. Chan1,
  2. Zhengang Peng2,3,
  3. Jennifer Chao Weber4,
  4. Ying-Wei Li2,3,
  5. Matthew T. Zuzolo2,3 and
  6. Huey-Jen L. Lin2,3,5

Published Online: 8 AUG 2012

DOI: 10.1002/9781118349045.ch22

Toxicology and Epigenetics

Toxicology and Epigenetics

How to Cite

Chan, M. W. Y., Peng, Z., Weber, J. C., Li, Y.-W., Zuzolo, M. T. and Lin, H.-J. L. (2012) Methods of Global Epigenomic Profiling, in Toxicology and Epigenetics (ed S. C. Sahu), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781118349045.ch22

Editor Information

  1. Division of Toxicology, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, Maryland, USA

Author Information

  1. 1

    Department of Life Science and Human Epigenomics Center, National Chung Cheng University, Min-Hsiung, Chia-Yi, Taiwan, ROC, Taiwan

  2. 2

    Division of Medical Technology, School of Allied Medical Professions, The Ohio State University, Columbus, OH, USA

  3. 3

    Molecular Biology and Cancer Genetics Program, College of Medicine, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA

  4. 4

    College of Medicine, University of Arizona, Phoenix, AZ, USA

  5. 5

    Department of Medical Technology, University of Delaware, Newark, DE, USA

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 7 SEP 2012

ISBN Information

Print ISBN: 9781119976097

Online ISBN: 9781118349045



  • methods of global epigenomic profiling;
  • epigenomic aberrations, embryonic and cell proliferation;
  • aberrant DNA methylation, captured by genomic profiling;
  • DMH capturing global changes in DNA methylation, at CpG islands;
  • hybridization-based gene chips/bisulfite, microarray chips in removing bias;
  • necessity of coupling NGS to genome-wide methylation profiling;
  • NGS technologies and issues of data storage, a major challenge;
  • taking NGS to a new era into epigenomic alterations, at lower cost


This chapter contains sections titled:

  • Introduction

  • DNA methylation

  • Histone modifications and chromatin remodeling

  • Noncoding RNA

  • Summary and discussion

  • Acknowledgments

  • References