7. Bridging Gaps: From Enzyme Discovery to Bioprocesses

  1. Daniela Gamenara1,
  2. Gustavo A. Seoane1,
  3. Patricia Saenz-Méndez2 and
  4. Pablo Domínguez de María3

Published Online: 24 SEP 2012

DOI: 10.1002/9781118409343.ch7

Redox Biocatalysis: Fundamentals and Applications

Redox Biocatalysis: Fundamentals and Applications

How to Cite

Gamenara, D., Seoane, G. A., Saenz-Méndez, P. and de María, P. D. (2012) Bridging Gaps: From Enzyme Discovery to Bioprocesses , in Redox Biocatalysis: Fundamentals and Applications, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118409343.ch7

Author Information

  1. 1

    Organic Chemistry Department, Facultad de Química, UdelaR, Montevideo, Uruguay

  2. 2

    Computational Chemistry and Biology Group, DETEMA and Organic Chemistry Department Facultad de Química, UdelaR Montevideo, Uruguay

  3. 3

    Institut für Technische und Madromolekulare Chemie (ITMC), RWTH Aachen University Aachen, Germany

Publication History

  1. Published Online: 24 SEP 2012
  2. Published Print: 12 OCT 2012

ISBN Information

Print ISBN: 9780470874202

Online ISBN: 9781118409343

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Keywords:

  • bridging gaps, enzyme discovery to bioprocesses;
  • enzyme identification, for desired reactions;
  • directed evolution and biocatalyst high-throughput-screening;
  • fluorescence formation for NAD(P)H detection;
  • metabolic engineering of baker's yeast, redox knock out/overexpression

Summary

This chapter contains sections titled:

  • Context

  • Enzyme Directed Evolution and High-Throughput-Screening of Biocatalysts

  • Successful Case: Baker's Yeast Redox Enzymes, Their Cloning, and Separate Overexpression

  • Whole-Cells vs. Isolated Enzymes: Medium Engineering

  • Beyond: Multistep Domino Biocatalytic Processes

  • Concluding Remarks

  • References