4. Design-Based Stereology in the Brain Bank Setting

  1. Peter R. Mouton PhD
  1. Mark W. Burke

Published Online: 22 NOV 2013

DOI: 10.1002/9781118444177.ch4

Neurostereology: Unbiased Stereology of Neural Systems

Neurostereology: Unbiased Stereology of Neural Systems

How to Cite

Burke, M. W. (2014) Design-Based Stereology in the Brain Bank Setting, in Neurostereology: Unbiased Stereology of Neural Systems (ed P. R. Mouton), John Wiley & Sons, Inc., Ames, USA. doi: 10.1002/9781118444177.ch4

Author Information

  1. Department of Physiology and Biophysics, College of Medicine, Howard University School of Medicine, Washington, DC, USA

Publication History

  1. Published Online: 22 NOV 2013
  2. Published Print: 17 JAN 2014

ISBN Information

Print ISBN: 9781118444214

Online ISBN: 9781118444177

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Keywords:

  • brain bank;
  • cortical neurons;
  • design-based stereology;
  • fetal alcohol exposure (FAE);
  • neuronal population;
  • vervet monkeys

Summary

This chapter provides a practical guide for design of a stereology project that can be modified to address their specific hypothesis. Using the example of a monkey brain study from the brain bank tissue, the chapter takes into consideration specimen preparation, design of a stereology project, and presentation of results. A two-step design-based approach begins with statistical sampling with systematic collection of serial histological sections through the reference space, followed by superimposing geometric probes to estimate the number or size of the particular cell population of interest. The chapter discusses a study on the practical application of design-based stereology. This study using vervet monkeys describes a recent series of experiments to study the neuroanatomical effects of naturalistic fetal alcohol exposure (FAE) on the neuronal population of the cortex. The findings indicate that relatively moderate and naturalistic alcohol consumption during the third trimester results in an overall loss of cortical neurons.