7. Order in Chaos: Stereological Studies of Nervous Tissue

  1. Peter R. Mouton PhD
  1. Peter Dockery

Published Online: 22 NOV 2013

DOI: 10.1002/9781118444177.ch7

Neurostereology: Unbiased Stereology of Neural Systems

Neurostereology: Unbiased Stereology of Neural Systems

How to Cite

Dockery, P. (2014) Order in Chaos: Stereological Studies of Nervous Tissue, in Neurostereology: Unbiased Stereology of Neural Systems (ed P. R. Mouton), John Wiley & Sons, Inc., Ames, USA. doi: 10.1002/9781118444177.ch7

Author Information

  1. Anatomy School of Medicine, National University of Ireland, Galway, Ireland

Publication History

  1. Published Online: 22 NOV 2013
  2. Published Print: 17 JAN 2014

ISBN Information

Print ISBN: 9781118444214

Online ISBN: 9781118444177

SEARCH

Keywords:

  • magnetic resonance imaging (MRI);
  • myelin sheath;
  • nervous system;
  • nervous tissue;
  • positron emission tomography (PET);
  • stereological studies

Summary

Medical imaging technologies such as magnetic resonance imaging (MRI), positron emission tomography (PET), ultrasonography, and optical imaging generate noninvasive, functionally relevant images that allow insight into structure-function relations in the living nervous system. This chapter illustrates the type of quantitative first- and second-order information on neural structural that can be obtained using unbiased sampling strategies in combination with simple morphometric and stereological probes. Through systematic-random sampling, the stereological approach gathers detailed quantitative information on structural parameters of neural tissue, including volume (3D), surface area (2D), length (1D), and number (0D), without assumptions about the geometrical properties of the structures. Average periodicity for a nerve may be expressed as the slope of the linear regression line of width of myelin sheath against number of lamellae. An example in the chapter examines unmyelinated axons in the tibial nerve of genetically diabetic mice C57BL/Ks (db/db) at 6 and 15 months of age.