4. Cell-Type Resolution Analysis of Root Development and Environmental Responses

  1. Martin Crespi
  1. José R. Dinneny1,2

Published Online: 10 OCT 2012

DOI: 10.1002/9781118447093.ch4

Root Genomics and Soil Interactions

Root Genomics and Soil Interactions

How to Cite

Dinneny, J. R. (2012) Cell-Type Resolution Analysis of Root Development and Environmental Responses, in Root Genomics and Soil Interactions (ed M. Crespi), Blackwell Publishing Ltd., Oxford, UK. doi: 10.1002/9781118447093.ch4

Editor Information

  1. Institut des Sciences du Vègètal, Centre National de la Recherche Scientifique, Gif sur Yvette cedex, France

Author Information

  1. 1

    Carnegie Institution for Science, Department of Plant Biology, Stanford, California, USA

  2. 2

    Temasek Lifesciences Laboratory, Department of Biological Sciences, National University of Singapore, Singapore

Publication History

  1. Published Online: 10 OCT 2012
  2. Published Print: 3 DEC 2012

ISBN Information

Print ISBN: 9780470960431

Online ISBN: 9781118447093

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Keywords:

  • root development cell-type resolution, and environmental responses;
  • gene expression patterns, cell-type-specific using in situ hybridization;
  • cell-type-specific gene expression in plant–environment interaction;
  • cell-type specification pathways, direct outcome of development;
  • cell-sorting methodology for Arabidopsis roots, three approaches;
  • FACS in cell-type-specific biology, and GFP expression;
  • LCM for in situ hybridization, for genome-scale information;
  • INTACT and nuclei isolation, advantages/disadvantages;
  • auxin, development gene expression pattern of PINs in cells;
  • salt stress/iron deprivation, regulatory pathways and CREs

Summary

This chapter contains sections titled:

  • Introduction

  • Tools for Cell-Type Resolution Analysis

  • Analysis of Spatiotemporal Expression Patterns in the Arabidopsis Root

  • Analysis of Cell-Type-Specific Expression Patterns in the Rice Root

  • Cell-Type-Specific Analysis of Auxin

  • Cell-Type-Specific Analyses of Chromatin

  • Cell-Type-Specific Analyses of Responses to Environmental Change

  • Future Prospects

  • Acknowledgments

  • References