7. Assessment of Bone Mass and Microarchitecture in Rodents

  1. Clifford J. Rosen MD
  1. Blaine A. Christiansen

Published Online: 19 JUL 2013

DOI: 10.1002/9781118453926.ch7

Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism, Eighth Edition

Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism, Eighth Edition

How to Cite

Christiansen, B. A. (2013) Assessment of Bone Mass and Microarchitecture in Rodents, in Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism, Eighth Edition (ed C. J. Rosen), John Wiley & Sons, Inc., Ames, USA. doi: 10.1002/9781118453926.ch7

Publication History

  1. Published Online: 19 JUL 2013

ISBN Information

Print ISBN: 9781118453889

Online ISBN: 9781118453926

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Keywords:

  • bone mass;
  • magnetic resonance imaging (MRI);
  • microarchitecture;
  • microcomputed tomography (μCT);
  • nanocomputed tomography (nCT);
  • peripheral dual-energy X-ray absorptiometry (pDXA);
  • peripheral quantitative computed tomography (pQCT);
  • radiographs;
  • rodents

Summary

This chapter reviews the imaging techniques commonly used to assess bone mass and microarchitecture in rodents, paying particular attention to their advantages and disadvantages, as well as technical challenges associated with each technique. It discusses imaging techniques, such as radiographs, peripheral dual-energy X-ray absorptiometry (pDXA), peripheral quantitative computed tomography (pQCT), magnetic resonance imaging (MRI) and nanocomputed tomography (nCT). Structural measurements obtained from microcomputed tomography (µCT) or other 3D imaging techniques are strongly dependent on a number of technical issues associated with the analysis, including (i) the scan resolution (voxel size), (ii) the segmentation algorithm and threshold used to delineate soft tissue from bone, (iii) the skeletal site(s) and volumes of interest, and (iv) calibration of the system using density phantoms. Assessment of skeletal mass and morphology in rodents via nondestructive imaging is an important component of current investigations aimed at improving our understanding of musculoskeletal development, growth, adaptation, and disease.