20. Separation and Purification of Lignocellulose Hydrolyzates

  1. Shri Ramaswamy2,
  2. Hua-Jiang Huang2 and
  3. Bandaru V. Ramarao3
  1. G. Peter van Walsum

Published Online: 4 MAR 2013

DOI: 10.1002/9781118493441.ch20

Separation and Purification Technologies in Biorefineries

Separation and Purification Technologies in Biorefineries

How to Cite

van Walsum, G. P. (2013) Separation and Purification of Lignocellulose Hydrolyzates, in Separation and Purification Technologies in Biorefineries (eds S. Ramaswamy, H.-J. Huang and B. V. Ramarao), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781118493441.ch20

Editor Information

  1. 2

    Department of Bioproducts and Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota, USA

  2. 3

    Department of Paper and Bioprocess Engineering, Empire State Paper Research Institute, State University of New York College of Environmental Science and Forestry, Syracuse, New York, USA

Author Information

  1. Forest Bioproducts Research Institute, Department of Chemical and Biological Engineering, University of Maine, Orono, Maine, USA

Publication History

  1. Published Online: 4 MAR 2013
  2. Published Print: 11 FEB 2013

ISBN Information

Print ISBN: 9780470977965

Online ISBN: 9781118493441



  • biomass detoxification;
  • biorefineries;
  • lignocellulose hydrolyzates;
  • purification technology;
  • separation technology


Producing sugars from lignocellulose is the most challenging but holds the greatest promise for the potential scale of the resource availability. Different simplified processing pathways for the sugar platform are illustrated in this chapter. The separation, purification, and detoxification of biomass hydrolyzates are of particular interest in this chapter. Several methods have been suggested for detoxification of pretreated or acid-hydrolyzed biomass, including: overliming, ammonium hydroxide, use of adsorbents such as activated carbon or polymeric adsorbents, solvent extraction, ion exchange, flocculation, treatment with microbes, treatment with enzymes. The sections describe some of the findings reported on these methods. Many methods of reducing the inhibitory nature of biomass hydrolysates have been developed, though all add cost and complexity to the operations of a biorefinery. The optimal solution will likely be specific to the biomass feedstock, downstream processing needs, and local opportunities for synergy with other biomass processing facilities.