15. Targeted Quantification – Selected Reaction Monitoring

  1. Ingvar Eidhammer,
  2. Harald Barsnes,
  3. Geir Egil Eide and
  4. Lennart Martens

Published Online: 10 JAN 2013

DOI: 10.1002/9781118494042.ch15

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry

How to Cite

Eidhammer, I., Barsnes, H., Eide, G. E. and Martens, L. (2013) Targeted Quantification – Selected Reaction Monitoring, in Computational and Statistical Methods for Protein Quantification by Mass Spectrometry, John Wiley & Sons Ltd, Oxford, UK. doi: 10.1002/9781118494042.ch15

Publication History

  1. Published Online: 10 JAN 2013
  2. Published Print: 4 JAN 2013

ISBN Information

Print ISBN: 9781119964001

Online ISBN: 9781118494042

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Keywords:

  • label based SRM based quantification;
  • label free SRM based quantification;
  • LC-MS/MS;
  • peptides;
  • selected ion chromatograms (SICs);
  • selected reaction monitoring (SRM);
  • SRM transitions;
  • targeted quantification method

Summary

This chapter describes the most common targeted quantification method, called Selected Reaction Monitoring (SRM). Mass spectrometry generally produces two levels of spectra: MS spectra and MS/MS spectra. The chapter also describes the specific mass spectrometer type commonly used for SRM, and how this instrument is used in the SRM runs. SRM requires the targeting of specific masses at both the MS and MS/MS level, requiring special instruments. The selected ion chromatograms (SICs) produced on the MS/MS level are used for quantification. Similar to the division used for discovery-oriented quantification, SRM based quantification can be divided into label free and label based approaches. Reliable quantification using SRM depends strongly on the selection of the transitions for the targeted proteins, thus highlighting the importance of selecting the best possible transitions. This in turn results in several requirements for both the peptides as well as their fragment ions.

Controlled Vocabulary Terms

recurrence quantification analysis