16. Absolute Quantification

  1. Ingvar Eidhammer,
  2. Harald Barsnes,
  3. Geir Egil Eide and
  4. Lennart Martens

Published Online: 10 JAN 2013

DOI: 10.1002/9781118494042.ch16

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry

How to Cite

Eidhammer, I., Barsnes, H., Eide, G. E. and Martens, L. (2013) Absolute Quantification, in Computational and Statistical Methods for Protein Quantification by Mass Spectrometry, John Wiley & Sons Ltd, Oxford, UK. doi: 10.1002/9781118494042.ch16

Publication History

  1. Published Online: 10 JAN 2013
  2. Published Print: 4 JAN 2013

ISBN Information

Print ISBN: 9781119964001

Online ISBN: 9781118494042

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Keywords:

  • absolute quantification;
  • label based absolute quantification;
  • label free absolute quantification;
  • MS/MS spectra;
  • peptides

Summary

The general procedure for absolute quantification is to use internal standards for which the absolute abundances are known. These standards can be peptides, a concatenation of peptides, or whole proteins, depending on the method used. This chapter discusses linear dependency between the calculated and the real abundances. Label based approaches are more accurate than label free approaches. The label based methods can be divided into three groups, depending on the type of standard used: stable isotope-labeled peptide standards, stable isotope-labeled concatenated peptide standards, and stable isotope-labeled intact protein standards. The label free methods use proteins as standards. These methods can be grouped depending on which spectra are used for quantification: MS spectra or MS/MS spectra.

Controlled Vocabulary Terms

Computational statistics; Inferential statistics