9. Label Based Quantification

  1. Ingvar Eidhammer,
  2. Harald Barsnes,
  3. Geir Egil Eide and
  4. Lennart Martens

Published Online: 10 JAN 2013

DOI: 10.1002/9781118494042.ch9

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry

Computational and Statistical Methods for Protein Quantification by Mass Spectrometry

How to Cite

Eidhammer, I., Barsnes, H., Eide, G. E. and Martens, L. (2013) Label Based Quantification, in Computational and Statistical Methods for Protein Quantification by Mass Spectrometry, John Wiley & Sons Ltd, Oxford, UK. doi: 10.1002/9781118494042.ch9

Publication History

  1. Published Online: 10 JAN 2013
  2. Published Print: 4 JAN 2013

ISBN Information

Print ISBN: 9781119964001

Online ISBN: 9781118494042

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Keywords:

  • label based quantification;
  • labeling techniques;
  • peptide variants;
  • reference based quantification;
  • reference free quantification

Summary

This chapter discusses the slightly simpler data analysis of the label based approaches. In label based quantification one typically examines two or more protein samples simultaneously in one quantification run. There are several ways to classify the various methods for labeled protein quantification. The ways are quantification level, label incorporation, incorporation level, number of compared samples, and common labels. The chapter examines that the quantification carried out at the MS level or on the MS/MS level. Intensities of the peaks corresponding to variants of the same peptide are then compared to obtain a relative quantification. Labels for MS/MS quantification are all chemically incorporated. Label based quantification can be performed with or without a reference sample, where the reference sample is used for experimental normalization.

Controlled Vocabulary Terms

recurrence quantification analysis