2. Next Generation Instruments and Methods for Proteomics

  1. Alejandro Cifuentes
  1. María del Carmen Mena and
  2. Juan Pablo Albar

Published Online: 4 MAR 2013

DOI: 10.1002/9781118537282.ch2

Foodomics: Advanced Mass Spectrometry in Modern Food Science and Nutrition

Foodomics: Advanced Mass Spectrometry in Modern Food Science and Nutrition

How to Cite

Mena, M. d. C. and Albar, J. P. (2013) Next Generation Instruments and Methods for Proteomics, in Foodomics: Advanced Mass Spectrometry in Modern Food Science and Nutrition (ed A. Cifuentes), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118537282.ch2

Editor Information

  1. Laboratory of Foodomics (CIAL), National Research Council (CSIC) Madrid, Spain

Author Information

  1. Functional Proteomics Group, Centro Nacional de Biotecnología–CSIC, Madrid, Spain

Publication History

  1. Published Online: 4 MAR 2013
  2. Published Print: 11 MAR 2013

ISBN Information

Print ISBN: 9781118169452

Online ISBN: 9781118537282

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Keywords:

  • Mass spectrometry;
  • quantitative proteomics;
  • targeted proteomics;
  • hybrid instruments;
  • bioinformatics tools

Summary

Proteomics techniques are powerful tools for a wide range of applications. In foodomics science, the new methods and available instruments with high sensitivity and specificity allow the confident assessment of proteins in different food samples, their bioactivity, as well as the study of contaminants in quality control analyses. The classical bottom-up and the increasingly popular top-down proteomics approaches provide a complementary accurate analysis of the samples. In addition, the move from shotgun to targeted proteomics that include multiple and selected reaction monitoring methods has conducted to a faster and more precise peptide and protein analysis. In order to quantify proteins, there are several methods of quantitative proteomics, such as differential stable isotope labeling and label-free techniques that are in some cases complementary and can be used to validate the methods and to obtain more confident results. In addition, there are several strategies to analyze the post-translational protein modification that have a great value in biological processes. On the other hand, it is necessary to use bioinformatics tools to process and analyze the huge amount of data obtained by proteomic techniques. All these topics will be covered in this chapter.