1. Confocal Laser Scanning Microscopy

  1. Jean-Pierre Goure
  1. Thomas Olivier and
  2. Baptiste Moine

Published Online: 25 APR 2013

DOI: 10.1002/9781118574386.ch1

Optics in Instruments: Applications in Biology and Medicine

Optics in Instruments: Applications in Biology and Medicine

How to Cite

Olivier, T. and Moine, B. (2013) Confocal Laser Scanning Microscopy, in Optics in Instruments: Applications in Biology and Medicine (ed J.-P. Goure), John Wiley & Sons, Inc., Hoboken, NJ USA. doi: 10.1002/9781118574386.ch1

Publication History

  1. Published Online: 25 APR 2013
  2. Published Print: 13 MAY 2013

ISBN Information

Print ISBN: 9781848212442

Online ISBN: 9781118574386

SEARCH

Keywords:

  • biology;
  • confocal laser scanning microscopy;
  • fluorescent labeling

Summary

This chapter attempts to lay the foundation for understanding the general principle of the optical method. Then it highlights some key elements of the current systems of confocal microscopy with emphasis on their role and operating principle. It offers a few illustrative examples to describe and discuss the advantages and limitations inherent in this method when used with biological media. It includes a brief introduction recalling some notions of biology and fluorescent labeling. Finally, the chapter briefly describes the main derived or related techniques. It focuses in particular on nonlinear contrast microscopy and concludes by referring to the latest advances that have enabled us to achieve optical resolutions below the diffraction limit.

Controlled Vocabulary Terms

biological techniques; near-field scanning optical microscopy; optical microscopes; optical scanners