10. Analytical Separations
Published Online: 7 JUN 2013
Copyright © 2013 John Wiley & Sons, Inc.
Basics of Analytical Chemistry and Chemical Equilibria
How to Cite
Tissue, B. M. (2013) Analytical Separations, in Basics of Analytical Chemistry and Chemical Equilibria, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118647042.ch10
- Published Online: 7 JUN 2013
- Published Print: 12 JUL 2013
Print ISBN: 9780470592083
Online ISBN: 9781118647042
- analytical separations;
- gas chromatography (GC);
- high performance liquid chromatography (HPLC);
- molecular mass spectrometry;
- thin-layer chromatography (TLC)
This chapter discusses operating principles and describes the most common instrumentation for methods for separating and quantitating analytes, namely, thin-layer chromatography (TLC), high performance liquid chromatography (HPLC), gas chromatography (GC) and electrophoresis. These separation methods are coupled to some type of detection or visualization, such as molecular mass spectrometry, to create an integrated system for qualitative and quantitative measurements. The power to separate and quantitate analytes simultaneously makes chromatographic instruments ubiquitous in analytical laboratories.