10. Analytical Separations

  1. Brian M. Tissue

Published Online: 7 JUN 2013

DOI: 10.1002/9781118647042.ch10

Basics of Analytical Chemistry and Chemical Equilibria

Basics of Analytical Chemistry and Chemical Equilibria

How to Cite

Tissue, B. M. (2013) Analytical Separations, in Basics of Analytical Chemistry and Chemical Equilibria, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118647042.ch10

Author Information

  1. Virginia Tech, Department of Chemistry, Blacksburg, VA, USA

Publication History

  1. Published Online: 7 JUN 2013
  2. Published Print: 12 JUL 2013

ISBN Information

Print ISBN: 9780470592083

Online ISBN: 9781118647042

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Keywords:

  • analytical separations;
  • electrophoresis;
  • gas chromatography (GC);
  • high performance liquid chromatography (HPLC);
  • molecular mass spectrometry;
  • thin-layer chromatography (TLC)

Summary

This chapter discusses operating principles and describes the most common instrumentation for methods for separating and quantitating analytes, namely, thin-layer chromatography (TLC), high performance liquid chromatography (HPLC), gas chromatography (GC) and electrophoresis. These separation methods are coupled to some type of detection or visualization, such as molecular mass spectrometry, to create an integrated system for qualitative and quantitative measurements. The power to separate and quantitate analytes simultaneously makes chromatographic instruments ubiquitous in analytical laboratories.