10. Frequently asked questions

  1. Geoff Daniels PhD, FRCPath Consultant Clinical Scientist and Head of Diagnostics1 and
  2. Imelda Bromilow MSc, CBiol Scientific Consultant2

Published Online: 6 SEP 2013

DOI: 10.1002/9781118688915.ch10

Essential Guide to Blood Groups, Third Edition

Essential Guide to Blood Groups, Third Edition

How to Cite

Daniels, G. and Bromilow, I. (eds) (2013) Frequently asked questions, in Essential Guide to Blood Groups, Third Edition, John Wiley & Sons, Oxford. doi: 10.1002/9781118688915.ch10

Editor Information

  1. 1

    IBGRL, Bristol Institute for Transfusion Services, NHS Blood and Transplant, Bristol, UK

  2. 2

    Liverpool, UK

Publication History

  1. Published Online: 6 SEP 2013
  2. Published Print: 3 SEP 2013

ISBN Information

Print ISBN: 9781118688922

Online ISBN: 9781118688915



  • alloimmunisation;
  • anti-D reagents;
  • blood grouping;
  • phenotypes;
  • pregnancy;
  • transfusion recipients


This conclusory chapter presents answers for various questions on blood grouping. Information provided includes differences between sensitivity and specificity, why anti-A,B is no longer obligatory in ABO typing and the reason for the recommendation of two anti-D reagents for RhD typing. The importance of detecting D variant (weak D and partial D) phenotypes is discussed. The chapter presents the reason for testing RhD positive women more than once during pregnancy. It also talks about how often should transfusion recipients be tested for the presence of antibodies and the differentiation of passive anti-D from anti-D due to alloimmunisation, the importance of antibody screening and presents reasons why a crossmatch by IAT is not enough to detect incompatible blood.