7. Blood grouping from DNA

  1. Geoff Daniels PhD, FRCPath Consultant Clinical Scientist and Head of Diagnostics1 and
  2. Imelda Bromilow MSc, CBiol Scientific Consultant2

Published Online: 6 SEP 2013

DOI: 10.1002/9781118688915.ch7

Essential Guide to Blood Groups, Third Edition

Essential Guide to Blood Groups, Third Edition

How to Cite

Daniels, G. and Bromilow, I. (eds) (2013) Blood grouping from DNA, in Essential Guide to Blood Groups, Third Edition, John Wiley & Sons, Oxford. doi: 10.1002/9781118688915.ch7

Editor Information

  1. 1

    IBGRL, Bristol Institute for Transfusion Services, NHS Blood and Transplant, Bristol, UK

  2. 2

    Liverpool, UK

Publication History

  1. Published Online: 6 SEP 2013
  2. Published Print: 3 SEP 2013

ISBN Information

Print ISBN: 9781118688922

Online ISBN: 9781118688915



  • cell-free fetal DNA;
  • human blood groups;
  • molecular methods;
  • next-generation sequencing (NGS);
  • serological methods


Almost all the genes for human blood groups have now been cloned and the molecular bases for all of the clinically important blood group polymorphisms determined. This chapter describes some applications of this technology and the methods applied. Cell-free fetal DNA derived from the placenta is detectable in the blood of pregnant women. Almost all laboratories applying this technology for diagnostic purposes employ real time quantitative polymerase chain reaction (PCR) with Taqman chemistry. Molecular tests can be used for testing patients and donors when serological reagents are of poor quality or in short supply. Molecular methods are already starting to replace serological methods for some blood group typing. Fetal genomes can also be sequenced from cell-free fetal DNA in the mother's blood by applying next-generation sequencing (NGS), so fetal blood grouping by NGS will also be developed soon.