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Rapid Scan, Stopped-Flow Kinetics

  1. Rui-Yong Wang

Published Online: 15 DEC 2011

DOI: 10.1002/9781119951438.eibc0302

Encyclopedia of Inorganic and Bioinorganic Chemistry

Encyclopedia of Inorganic and Bioinorganic Chemistry

How to Cite

Wang, R.-Y. 2011. Rapid Scan, Stopped-Flow Kinetics. Encyclopedia of Inorganic and Bioinorganic Chemistry. .

Author Information

  1. Zhengzhou University, Zhengzhou, China

Publication History

  1. Published Online: 15 DEC 2011

Abstract

One of the most frequently used rapid kinetics techniques is the stopped-flow method. A major strength of rapid mixing methods is that they can be readily combined with a wide range of detection methods. Applications of stopped-flow techniques, mainly stopped-flow absorbance and fluorescence spectrometry, in the fields of inorganic and bioinorganic chemistry include kinetic measurement of ligand substitution, ligand binding and dissociation, redox reactions, and so on. The kinetic and thermodynamic parameters can be calculated with the aid of stopped-flow methods. The general procedures for kinetic and thermodynamic studies by using stopped-flow techniques are also discussed.

Keywords:

  • stopped-flow;
  • rapid-scan;
  • spectroscopy;
  • fluorescence spectroscopy;
  • kinetics;
  • mechanism