Rapid Scan, Stopped-Flow Kinetics
Published Online: 15 DEC 2011
Copyright © 2011 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Inorganic and Bioinorganic Chemistry
How to Cite
Wang, R.-Y. 2011. Rapid Scan, Stopped-Flow Kinetics. Encyclopedia of Inorganic and Bioinorganic Chemistry. .
- Published Online: 15 DEC 2011
One of the most frequently used rapid kinetics techniques is the stopped-flow method. A major strength of rapid mixing methods is that they can be readily combined with a wide range of detection methods. Applications of stopped-flow techniques, mainly stopped-flow absorbance and fluorescence spectrometry, in the fields of inorganic and bioinorganic chemistry include kinetic measurement of ligand substitution, ligand binding and dissociation, redox reactions, and so on. The kinetic and thermodynamic parameters can be calculated with the aid of stopped-flow methods. The general procedures for kinetic and thermodynamic studies by using stopped-flow techniques are also discussed.
- fluorescence spectroscopy;