Standard Article


  1. Kevin D Swanson,
  2. Danillo O Ortillo,
  3. Joan B Broderick,
  4. John W Peters

Published Online: 15 JUN 2012

DOI: 10.1002/9781119951438.eibc2055

Encyclopedia of Inorganic and Bioinorganic Chemistry

Encyclopedia of Inorganic and Bioinorganic Chemistry

How to Cite

Swanson, K. D., Ortillo, D. O., Broderick, J. B. and Peters, J. W. 2012. [FeFe]-Hydrogenase. Encyclopedia of Inorganic and Bioinorganic Chemistry. .

Author Information

  1. Montana State University, Department of Chemistry and Biochemistry, Astrobiology Biogeocatalysis Research Center, Bozeman, USA

Publication History

  1. Published Online: 15 JUN 2012


[FeFe]-Hydrogenases are metalloenzymes that are key components of the energy metabolism in many microbial communities. Hydrogenases function either to couple hydrogen oxidation to energy yielding processes or in proton reduction as a means to generate oxidized electron carriers required in fermentation. These enzymes have complex metal cluster with unique nonprotein derived ligands and are of significant interest due to their complexity and novel features as well as in biotechnology in developing superior hydrogen production methods. Biochemical, structural, and spectroscopic studies have provided significant insights into how these enzymes work and how these complex metal clusters key to their function are synthesized. This short report highlights some of the key features of the enzymes that have been elucidated through many years and the recent progress on [FeFe]-hydrogenase maturation pathway and synthesis of the active site H cluster.

3D Structure


  • FeFe-hydrogenase;
  • H-cluster;
  • hydrogen;
  • cyanide CN;
  • carbon monoxide CO;
  • dithiolate;
  • Fe–S cluster;
  • HydE;
  • HydF;
  • HydG