Precursor-Bound NifEN: Insights into Nitrogenase Cofactor Assembly
Published Online: 15 SEP 2012
Copyright © 2011 John Wiley & Sons, Ltd. All rights reserved.
Encyclopedia of Inorganic and Bioinorganic Chemistry
How to Cite
Wiig, J. A., Lee, C. C., Hu, Y. and Ribbe, M. W. 2012. Precursor-Bound NifEN: Insights into Nitrogenase Cofactor Assembly. Encyclopedia of Inorganic and Bioinorganic Chemistry. .
- Published Online: 15 SEP 2012
The biosynthesis of the iron–molybdenum cofactor (FeMoco) of nitrogenase is one of the most complicated processes in metalloprotein biochemistry. Here we review the recent progress on the investigation of NifEN, an important scaffold protein for the maturation of an iron-only precursor to a molybdenum- and homocitrate-containing FeMoco. The recently solved crystal structure of the precursor-bound form of NifEN is discussed in detail, which provides important insights into the biosynthetic mechanism of nitrogenase. In addition, the functional and spectroscopic features of NifEN are discussed.
- Mo nitrogenase;
- MoFe protein;
- Fe protein;
- Azotobacter vinelandii