24. Crosslinked and Polymerized Hemoglobins as Potential Blood Substitutes

  1. Andrea Mozzarelli2,3 and
  2. Stefano Bettati2,3
  1. Kenneth W. Olsen and
  2. Eugene Tarasov

Published Online: 4 JUL 2011

DOI: 10.1002/9781119975427.ch24

Chemistry and Biochemistry of Oxygen Therapeutics: From Transfusion to Artificial Blood

Chemistry and Biochemistry of Oxygen Therapeutics: From Transfusion to Artificial Blood

How to Cite

Olsen, K. W. and Tarasov, E. (2011) Crosslinked and Polymerized Hemoglobins as Potential Blood Substitutes, in Chemistry and Biochemistry of Oxygen Therapeutics: From Transfusion to Artificial Blood (eds A. Mozzarelli and S. Bettati), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781119975427.ch24

Editor Information

  1. 2

    Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy

  2. 3

    National Institute of Biostructures and Biosystems, Rome, Italy

Author Information

  1. Department of Chemistry, Loyola University, Chicago, IL, USA

Publication History

  1. Published Online: 4 JUL 2011
  2. Published Print: 12 AUG 2011

ISBN Information

Print ISBN: 9780470686683

Online ISBN: 9781119975427

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Keywords:

  • crosslinked and polymerized hemoglobins - as potential blood substitutes;
  • HBOC production, modification of hemoglobin (Hb) tetramer - properties for blood substitute;
  • crosslinking Hb, logical step - in avoiding Hb extravasation into tissue;
  • double crosslinking of protein - Hb, crosslinking followed by PEGylation;
  • multilinker structures, and multilinking - three or more groups on Hb;
  • multilinker success - and tetralinked Hb octamer production;
  • Hb octamer formation - using azide–alkyne click chemistry;
  • Hb polymers, large Hb oligomer development - using polymerization methods;
  • polymerization, by sulfhydryl–maleimide click chemistry;
  • crosslinking tetramer before polymerization - stabilizing protein controlling oxygen affinity

Summary

This chapter contains sections titled:

  • Introduction

  • Crosslinking the Hb Tetramer

  • Hb Polymers

  • Conclusion

  • References