26. Hb Octamers by Introduction of Surface Cysteines

  1. Andrea Mozzarelli3,4 and
  2. Stefano Bettati3,4
  1. Véronique Baudin-Creuza1,
  2. Chien Ho2 and
  3. Michael C. Marden1

Published Online: 4 JUL 2011

DOI: 10.1002/9781119975427.ch26

Chemistry and Biochemistry of Oxygen Therapeutics: From Transfusion to Artificial Blood

Chemistry and Biochemistry of Oxygen Therapeutics: From Transfusion to Artificial Blood

How to Cite

Baudin-Creuza, V., Ho, C. and Marden, M. C. (2011) Hb Octamers by Introduction of Surface Cysteines, in Chemistry and Biochemistry of Oxygen Therapeutics: From Transfusion to Artificial Blood (eds A. Mozzarelli and S. Bettati), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781119975427.ch26

Editor Information

  1. 3

    Department of Biochemistry and Molecular Biology, University of Parma, Parma, Italy

  2. 4

    National Institute of Biostructures and Biosystems, Rome, Italy

Author Information

  1. 1

    Inserm U779, CHU Bicêtre, Paris, France

  2. 2

    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA

Publication History

  1. Published Online: 4 JUL 2011
  2. Published Print: 12 AUG 2011

ISBN Information

Print ISBN: 9780470686683

Online ISBN: 9781119975427

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Keywords:

  • Hb octamers - and surface cysteine introduction;
  • RBC autocatalytic degradation;
  • genetic engineering of proteins - with cysteines;
  • Hb octamer properties - and point mutations of Hb;
  • naturally occurring cysteines - in human Hb;
  • Hbs expressed in E. coli - in a reducing environment;
  • recombinant Hbs - with additional cysteines;
  • test for perspective blood substitute - octamer stability, in fresh plasma presence;
  • factors, hindering HBOC - oxygen affinity, oxidation rate, a problem;
  • recombinant technology for Hb, short supply - and novel molecules, as real blood substitutes

Summary

This chapter contains sections titled:

  • Introduction

  • Genetic Engineering of Proteins with Cysteines

  • Conclusion

  • References