11. Biomarker Detection and Molecular Profiling by Multiplex Microbead Suspension Array Based Immunoproteomics

  1. Ralph Rapley4 and
  2. Stuart Harbron5
  1. V. V. Krishhan1,
  2. Imran H. Khan2 and
  3. Paul A. Luciw3

Published Online: 7 OCT 2011

DOI: 10.1002/9781119977438.ch11

Molecular Analysis and Genome Discovery, Second Edition

Molecular Analysis and Genome Discovery, Second Edition

How to Cite

Krishhan, V. V., Khan, I. H. and Luciw, P. A. (2011) Biomarker Detection and Molecular Profiling by Multiplex Microbead Suspension Array Based Immunoproteomics, in Molecular Analysis and Genome Discovery, Second Edition (eds R. Rapley and S. Harbron), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781119977438.ch11

Editor Information

  1. 4

    University of Hertfordshire, UK

  2. 5

    The Enzyme Technology Consultancy, UK

Author Information

  1. 1

    Department of Chemistry, California State University, Fresno CA 93740 and Center for Comparative Medicine, and Department of Pathology and Laboratory Medicine, University of California, Davis, CA 95616, USA

  2. 2

    Center for Comparative Medicine, and Department of Pathology and Laboratory Medicine, University of California, Davis CA 95616, USA

  3. 3

    Center for Comparative Medicine, Department of Pathology and Laboratory Medicine, and California National Primate Research Center, University of California, Davis, CA 95616, USA

Publication History

  1. Published Online: 7 OCT 2011
  2. Published Print: 4 NOV 2011

ISBN Information

Print ISBN: 9780470758779

Online ISBN: 9781119977438

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Keywords:

  • biomarker detection and molecular profiling;
  • microbead suspension array based immunoassays;
  • green yttrium aluminum garnet (YAG) laser;
  • flow cytometer-based approach;
  • microbead sandwich immunoassay;
  • disease-modifying antirheumatic drugs (DMARDs);
  • Luminex based assays;
  • automation;
  • particle (microbead)-based flow cytometric assays

Summary

This chapter contains sections titled:

  • Introduction

  • Principles of microbead-based multiplexing

  • Experimental aspects of the multiplex microbead assay

  • Multiplex microbead assay design and comparison with other methods

  • Applications of the multiplex microbead assay system for biomedical research and clinical studies

  • Selected investigational fields for multiplex analysis and examples of applications

  • Challenges and current limitations

  • Summary and future directions

  • Acknowledgements

  • References