3. qPCR, Theory, Reliability and Use in Molecular Analysis

  1. Ralph Rapley2 and
  2. Stuart Harbron3
  1. Jamie Murphy and
  2. Stephen A. Bustin

Published Online: 7 OCT 2011

DOI: 10.1002/9781119977438.ch3

Molecular Analysis and Genome Discovery, Second Edition

Molecular Analysis and Genome Discovery, Second Edition

How to Cite

Murphy, J. and Bustin, S. A. (2011) qPCR, Theory, Reliability and Use in Molecular Analysis, in Molecular Analysis and Genome Discovery, Second Edition (eds R. Rapley and S. Harbron), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9781119977438.ch3

Editor Information

  1. 2

    University of Hertfordshire, UK

  2. 3

    The Enzyme Technology Consultancy, UK

Author Information

  1. Academic Surgical Unit, 3rd Floor Alexandra Wing, Royal London Hospital, Whitechapel, London E1 1BB, UK

Publication History

  1. Published Online: 7 OCT 2011
  2. Published Print: 4 NOV 2011

ISBN Information

Print ISBN: 9780470758779

Online ISBN: 9781119977438

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Keywords:

  • qPCR in molecular analysis;
  • real-time reverse-transcription polymerase chain reaction;
  • laser capture microdissection (LCM);
  • total RNA extract quality;
  • target-specific standard curves;
  • assay design;
  • prime cDNA synthesis;
  • transcript detection;
  • threshold cycle (Cq) parameter;
  • RT-qPCR assays

Summary

This chapter contains sections titled:

  • Sample preparation

  • RNA quality

  • Reagents

  • Assay design

  • Transparency of published data

  • Further considerations

  • Conclusion

  • References