Standard Article

Carrot

Part 6. Transgenic Vegetable Crops

  1. Nina L. McCormick1,
  2. Brendan Rodoni2,
  3. Paul Taylor3,
  4. Rebecca Ford3

Published Online: 15 APR 2009

DOI: 10.1002/9781405181099.k0606

Compendium of Transgenic Crop Plants

Compendium of Transgenic Crop Plants

How to Cite

McCormick, N. L., Rodoni, B., Taylor, P. and Ford, R. 2009. Carrot. Compendium of Transgenic Crop Plants. 6:6:135–144.

Author Information

  1. 1

    Florigene Ltd Pty, Victoria, Australia

  2. 2

    Department of Primary Industries, Knoxfield, Victoria, Australia

  3. 3

    University of Melbourne, BioMarka, Parkville, Victoria, Australia

Publication History

  1. Published Online: 15 APR 2009

Abstract

The development of embryolike structures from aseptically cultured carrot cells was first demonstrated almost 50 years ago, and from these cultured cells entire plantlets were regenerated. This in vitro culture of carrot cells has become increasingly important for the production of transgenic plant lines where naturally available elite backgrounds are unavailable. Optimizing the Agrobacterium–plant interaction is considered to be the most important aspect for the development of an efficient Agrobacterium-mediated transformation protocol, together with the subsequent recovery of whole transgenic plants. The introduction of foreign DNA into plant cells has been widely applied for the genetic manipulation of carrot phenotypes and the development of cultivars with traits of commercial interest.

Keywords:

  • Daucus carota;
  • somatic embryogenesis;
  • Agrobacterium-mediated transformation