Chapter 10. Genetic Manipulation of Hematopoietic Stem Cells

  1. Frederick R. Appelbaum MD Member Director Professor Head3,4,
  2. Stephen J. Forman MD Director Staff Physician5,6,
  3. Robert S. Negrin MD Professor of Medicine Chief7 and
  4. Karl G. Blume MD, FACP Emeritus Professor of Medicine7,8
  1. Grant D. Trobridge PhD1 and
  2. Hans-Peter Kiem MD Member Professor of Medicine2

Published Online: 5 MAR 2009

DOI: 10.1002/9781444303537.ch10

Thomas' Hematopoietic Cell Transplantation: Stem Cell Transplantation, Fourth Edition

Thomas' Hematopoietic Cell Transplantation: Stem Cell Transplantation, Fourth Edition

How to Cite

Trobridge, G. D. and Kiem, H.-P. (2009) Genetic Manipulation of Hematopoietic Stem Cells, in Thomas' Hematopoietic Cell Transplantation: Stem Cell Transplantation, Fourth Edition (eds F. R. Appelbaum, S. J. Forman, R. S. Negrin and K. G. Blume), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444303537.ch10

Editor Information

  1. 3

    Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA

  2. 4

    Division of Medical Oncology, University of Washington School of Medicine, Seattle, Washington, USA

  3. 5

    Department of Hematology and Hematopoietic Cell Transplantation, Duarte, California, USA

  4. 6

    Department of Medical Oncology and Therapeutics Research, City of Hope Comprehensive Cancer Center, Duarte, California, USA

  5. 7

    Division of Blood and Marrow Transplantation, Stanford University, Stanford, California, USA

  6. 8

    Division of Bone Marrow Transplantation, Department of Medicine, Stanford University, School of Medicine, Stanford, California, USA

Author Information

  1. 1

    Fred Hutchinson Cancer Research Center, Seattle, WA, USA

  2. 2

    Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington, USA

Publication History

  1. Published Online: 5 MAR 2009
  2. Published Print: 27 FEB 2009

ISBN Information

Print ISBN: 9781405153485

Online ISBN: 9781444303537

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Keywords:

  • genetic manipulation of hematopoietic stem cells;
  • gammaretroviral vectors;
  • gene transfer vectors;
  • retroviral pseudotypes for HSC gene transfer;
  • expansion of gene-marked cells in vivo;
  • chronic granulomatous disease;
  • ADA deficiency;
  • vector-mediated insertional mutagenesis;
  • deregulation of host genes by vector proviruses

Summary

This chapter contains sections titled:

  • Introduction and history

  • Gene transfer vectors

  • Gene transfer to HSCs

  • Clinical trials of HSC gene transfer

  • Vector-mediated insertional mutagenesis

  • Summary and future directions

  • References