Chapter 5. Oligosaccharides

  1. J. M. Rosenfeld
  1. Hélène Perreault

Published Online: 3 MAR 2009

DOI: 10.1002/9781444305500.ch5

Sample Preparation for Hyphenated Analytical Techniques

Sample Preparation for Hyphenated Analytical Techniques

How to Cite

Perreault, H. (2004) Oligosaccharides, in Sample Preparation for Hyphenated Analytical Techniques (ed J. M. Rosenfeld), Blackwell Publishing Ltd., Oxford, UK. doi: 10.1002/9781444305500.ch5

Editor Information

  1. Faculty of Health Science, McMaster University, 1200 Main Street W, Hamilton, Ontario, Canada L8N 3Z5

Author Information

  1. Department of Chemistry, Room 517 Parker Building, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2

Publication History

  1. Published Online: 3 MAR 2009
  2. Published Print: 27 SEP 2004

ISBN Information

Print ISBN: 9781405111065

Online ISBN: 9781444305500



  • oligosaccharides;
  • high performance liquid chromatography/electrospray ionization–mass spectrometry (HPLC/ESI-MS);
  • direct-deposition matrix assisted laser desorption/ionization–mass spectrometry (MALDI MS);
  • electron impact ionization (EI) and chemical ionization (CI);
  • glycoprotein purification and molecular mass measurement;
  • oligosaccharides from other biological sources;
  • milk oligosaccharides;
  • carbohydrate-containing compounds of diverse categories;
  • sodium dodecyl sulfate (SDS) PAGE;
  • on-line and off-line hyphenation of separation techniques - tool in glycobiology


This chapter contains sections titled:

  • Introduction

  • Oligosaccharides from glycoproteins

  • Oligosaccharides from other biological sources

  • Methods

  • Summary and general considerations

  • Acknowledgements

  • References