Chapter 39. Haemopoietic Stem Cell Processing and Storage

  1. Michael F. Murphy MD, FRCP, FRCPath Professor Consultant Haematologist4,5 and
  2. Derwood H. Pamphilon MD, MRCPCH, FRCP, FRCPath Consultant Haematologist Honorary Clinical Reader6,7
  1. David H. McKenna Jr MD Assistant Professor Medical Director1,2,3 and
  2. Mary E. Clay MS, MT (ASCP)3

Published Online: 5 MAY 2009

DOI: 10.1002/9781444311761.ch39

Practical Transfusion Medicine, Third Edition

Practical Transfusion Medicine, Third Edition

How to Cite

McKenna, D. H. and Clay, M. E. (2009) Haemopoietic Stem Cell Processing and Storage, in Practical Transfusion Medicine, Third Edition (eds M. F. Murphy and D. H. Pamphilon), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444311761.ch39

Editor Information

  1. 4

    University of Oxford, Oxford, UK

  2. 5

    NHS Blood and Transplant and Department of Haematology, John Radcliffe Hospital, Oxford, UK

  3. 6

    NHS Blood and Transplant, Bristol, UK

  4. 7

    Department of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

Author Information

  1. 1

    University of Minnesota Medical School, Minneapolis, Minnesota, USA

  2. 2

    Molecular and Cellular Therapeutics, Minneapolis/Saint Paul, Minnesota, USA

  3. 3

    Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota, USA

Publication History

  1. Published Online: 5 MAY 2009
  2. Published Print: 14 APR 2009

ISBN Information

Print ISBN: 9781405181969

Online ISBN: 9781444311761

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Keywords:

  • haemopoietic stem cell (HSC) transplantation;
  • sources of HSCs – bone marrow, peripheral blood and umbilical cord blood;
  • haemopoietic growth factors;
  • post-allogeneic HSC transplant therapy;
  • human leucocyte antigen (HLA);
  • volume reduction (VR);
  • red blood cell depletion;
  • counterflow centrifugal elutriation;
  • monoclonal antibody-based technologies

Summary

This chapter contains sections titled:

  • Sources of HSCs

  • Processing methods

  • Routine methods

  • Specialised methods

  • Quality control testing techniques

  • Cryopreservation and storage

  • Quality assurance and good manufacturing practices

  • Conclusions

  • Key points

  • Further reading