Chapter 5. Phenotyping of Genetically Modified Mice

  1. Robert Hubrecht and
  2. James Kirkwood
  1. Rikke Westh Thon1,
  2. Merel Ritskes-hoitinga2,
  3. Hilary Gates3 and
  4. Jan-bas Prins4

Published Online: 8 FEB 2010

DOI: 10.1002/9781444318777.ch5

The UFAW Handbook on the Care and Management of Laboratory and Other Research Animals, Eighth Edition

The UFAW Handbook on the Care and Management of Laboratory and Other Research Animals, Eighth Edition

How to Cite

Westh Thon, R., Ritskes-hoitinga, M., Gates, H. and Prins, J.-b. (2010) Phenotyping of Genetically Modified Mice, in The UFAW Handbook on the Care and Management of Laboratory and Other Research Animals, Eighth Edition (eds R. Hubrecht and J. Kirkwood), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444318777.ch5

Editor Information

  1. UFAW, The Old School, Brewhouse Hill, Wheathampstead, Hertfordshire AL4 8AN, UK

Author Information

  1. 1

    PixieGene A/S, Axelborg, Copenhagen, Denmark

  2. 2

    Head Central Animal Laboratory, 231 Centraal Dierenlaboratorium (CDL), Universitair Medisch Centrum (UMC) St Radboud, PO Box 9101, NL-6500 HB Nijmegen, The Netherlands

  3. 3

    MRC, Mammalian Genetics Unit, Harwell OX11 0RD, UK

  4. 4

    Leiden University Medical Center, Leiden, The Netherlands

Publication History

  1. Published Online: 8 FEB 2010
  2. Published Print: 26 MAR 2010

ISBN Information

Print ISBN: 9781405175234

Online ISBN: 9781444318777

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Keywords:

  • phenotyping of genetically modified mice;
  • new genetically modified mouse - potential new strain;
  • welfare of genetically modified animals;
  • methods of welfare assessment - 1995 EU workshop, indices of good and bad welfare;
  • Mouse Phenome Database (MPD) - phenotypes from inbred mouse strains;
  • European Mouse Disease Clinic EUMODIC - primary phenotype assessment, 650 mouse mutant lines;
  • commercial phenotyping by companies - offering service to customers;
  • German Mouse Clinic (GMC) at GSF, Munich - open platform for phenotyping;
  • new methods for phenotyping - speed and sophistication;
  • testing every new strain in three stages - young, adult and geriatric

Summary

This chapter contains sections titled:

  • Introduction

  • Welfare issues

  • Other advantages of phenotyping

  • Before phenotyping – search for information

  • The dilemma of large-scale versus ‘home’ phenotyping

  • Testing for new traits – the hierarchical approach

  • Further phenotyping issues to consider

  • The environment – a challenge to phenotyping

  • Useful websites

  • Summary of phenotyping issues to consider

  • Concluding remarks

  • References