Chapter 19. Rapid Methods for the Identification of Seafood Micro-Organisms

  1. Associate Professor Cesarettin Alasalvar2,
  2. Professor Fereidoon Shahidi3,
  3. Professor Kazuo Miyashita4 and
  4. Dr Udaya Wanasundara5
  1. Brian H. Himelbloom,
  2. Alexandra C. M. Oliveira and
  3. Thombathu S. Shetty

Published Online: 2 SEP 2010

DOI: 10.1002/9781444325546.ch19

Handbook of Seafood Quality, Safety and Health Applications

Handbook of Seafood Quality, Safety and Health Applications

How to Cite

Himelbloom, B. H., Oliveira, A. C. M. and Shetty, T. S. (2010) Rapid Methods for the Identification of Seafood Micro-Organisms, in Handbook of Seafood Quality, Safety and Health Applications (eds C. Alasalvar, F. Shahidi, K. Miyashita and U. Wanasundara), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444325546.ch19

Editor Information

  1. 2

    TÜBİTAK Marmara Research Centre, Food Institute, Gebze/Kocaeli, Turkey

  2. 3

    Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada

  3. 4

    Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan

  4. 5

    POS Pilot Plant Corporation, Saskatoon, SK, Canada

Author Information

  1. Fishery Industrial Technology Center, School of Fisheries and Ocean Sciences, University of Alaska Fairbanks, Kodiak, AK, USA

Publication History

  1. Published Online: 2 SEP 2010
  2. Published Print: 1 OCT 2010

ISBN Information

Print ISBN: 9781405180702

Online ISBN: 9781444325546



  • rapid methods, identification of seafood micro-organisms;
  • bacteria, important role in quality and safety of seafood;
  • identifying bacteria, allowing analysts - to describe changing microflora composition to predict shelf-life;
  • carbon utilization, phenotypic characterization of micro-organisms - and commercially-available system;
  • Microbial Identification Inc. (MIDI), for identifying spoilage bacteria during iced storage of Alaska pink salmon;
  • standardized identification systems - designed for microbiology laboratories;
  • molecular (genotyping) - polymerase chain reaction (PCR) and real-time or quantitative PCR (qPCR);
  • molecular subtyping techniques - denaturing gradient gel electrophoresis (DGGE), TTGE and single-strand conformation polymorphism (SSCP) - the latest innovations;
  • polyphasic taxonomy principles - combining phenotyping and genotyping;
  • time, cost, and advanced technical training - major factors controlling identification techniques


This chapter contains sections titled:

  • Introduction

  • Non-molecular (phenotyping)

  • Molecular (genotyping)

  • Conclusions

  • Acknowledgements

  • References