Chapter 23. Molecular Detection of Pathogens in Seafood

  1. Associate Professor Cesarettin Alasalvar2,
  2. Professor Fereidoon Shahidi3,
  3. Professor Kazuo Miyashita4 and
  4. Dr Udaya Wanasundara5
  1. Iddya Karunasagar and
  2. Indrani Karunasagar

Published Online: 2 SEP 2010

DOI: 10.1002/9781444325546.ch23

Handbook of Seafood Quality, Safety and Health Applications

Handbook of Seafood Quality, Safety and Health Applications

How to Cite

Karunasagar, I. and Karunasagar, I. (2010) Molecular Detection of Pathogens in Seafood, in Handbook of Seafood Quality, Safety and Health Applications (eds C. Alasalvar, F. Shahidi, K. Miyashita and U. Wanasundara), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444325546.ch23

Editor Information

  1. 2

    TÜBİTAK Marmara Research Centre, Food Institute, Gebze/Kocaeli, Turkey

  2. 3

    Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada

  3. 4

    Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan

  4. 5

    POS Pilot Plant Corporation, Saskatoon, SK, Canada

Author Information

  1. Department of Fishery Microbiology, KVAFSU, College of Fisheries, Mangalore, India

Publication History

  1. Published Online: 2 SEP 2010
  2. Published Print: 1 OCT 2010

ISBN Information

Print ISBN: 9781405180702

Online ISBN: 9781444325546



  • molecular detection of pathogens in seafood;
  • seafood safety management, information on presence/level of pathogens at different stages of food chain;
  • Food and Drug Administration (FDA) Guidance (Compliance Programme 7303.842) for Vibrio parahaemolyticus;
  • DNA probe hybridization based colorimetric assay Listeria GENE-TRAK - as Official Method 993.09 of Association of Official Analytical Chemists (AOAC);
  • nucleic acid amplification methods - bacterial pathogen detection;
  • L. monocytogenes, pathogen of great concern - in seafood safety management;
  • viral pathogen detection;
  • Real-time PCR assays - Real-time PCR (quantitative PCR or qPCR) - for detection of food-borne pathogens;
  • use of DNA microarray technology - for food-borne pathogen study;
  • molecular methods - testing for compliance with microbiological criteria or HACCP measures


This chapter contains sections titled:

  • Introduction

  • Probe hybridisation methods

  • Nucleic acid amplification methods

  • Conclusions

  • References