Chapter 24. DNA-Based Detection of Commercial Fish Species

  1. Associate Professor Cesarettin Alasalvar3,
  2. Professor Fereidoon Shahidi4,
  3. Professor Kazuo Miyashita5 and
  4. Dr Udaya Wanasundara6
  1. Rosalee S. Rasmussen1 and
  2. Michael T. Morrissey2

Published Online: 2 SEP 2010

DOI: 10.1002/9781444325546.ch24

Handbook of Seafood Quality, Safety and Health Applications

Handbook of Seafood Quality, Safety and Health Applications

How to Cite

Rasmussen, R. S. and Morrissey, M. T. (2010) DNA-Based Detection of Commercial Fish Species, in Handbook of Seafood Quality, Safety and Health Applications (eds C. Alasalvar, F. Shahidi, K. Miyashita and U. Wanasundara), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444325546.ch24

Editor Information

  1. 3

    TÜBİTAK Marmara Research Centre, Food Institute, Gebze/Kocaeli, Turkey

  2. 4

    Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada

  3. 5

    Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan

  4. 6

    POS Pilot Plant Corporation, Saskatoon, SK, Canada

Author Information

  1. 1

    Seafood Laboratory, Department of Food Science and Technology, Oregon State University, Astoria, OR, USA

  2. 2

    Food Innovation Centre, Oregon State University, Portland, OR, USA

Publication History

  1. Published Online: 2 SEP 2010
  2. Published Print: 1 OCT 2010

ISBN Information

Print ISBN: 9781405180702

Online ISBN: 9781444325546



  • DNA-based detection of commercial fish species;
  • international growth in trade and consumption of fish - increased potential for species substitution or mislabelling;
  • protein analysis, as isoelectric focusing (IEF) and immunoassays;
  • DNA-based methods and gene targets;
  • PCR-AFLP, technique involving aspects - of both PCR-RFLP and PCR-RAPD;
  • multiplex PCR, adapted for use with real-time PCR;
  • appropriate gene target - for species identification involving several factors;
  • fish species substitution, worldwide problem - in terms of domestic and international trade;
  • use of DNA barcodes - identifying Canadian freshwater fish in context of FISH-BOL;
  • DNA-based techniques, detection of commercial fish species substitution - and traditional sequencing


This chapter contains sections titled:

  • Introduction

  • DNA-based methods and gene targets

  • Major collaborative efforts

  • Conclusions

  • Acknowledgements

  • References