Two. Diagnostic Procedures and Principles of Commonly Used Tests

  1. Barbara J. Bain MB BS, FRACP, FRCPath Professor of Diagnostic Haematology1,
  2. Barbara J. Wild PhD, FIBMS Clinical Scientist2,
  3. Adrian D. Stephens MB BS, MD, FRCPath Honorary Consultant Haematologist3,
  4. Lorraine A. Phelan MIBMS4

Published Online: 18 NOV 2010

DOI: 10.1002/9781444328318.ch2

Book Title

Variant Haemoglobins: A Guide to Identification

Variant Haemoglobins: A Guide to Identification

Additional Information

How to Cite

Bain, B. J., Wild, B. J., Stephens, A. D. and Phelan, L. A. (2010) Diagnostic Procedures and Principles of Commonly Used Tests, in Variant Haemoglobins: A Guide to Identification, Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444328318.ch2

Author Information

  1. 1

    St Mary's Hospital Campus of Imperial College Faculty of Medicine, St Mary's Hospital, London, UK

  2. 2

    Haemoglobinopathy Genetics Centre, University College Hospitals London NHS Foundation Trust, London, UK

  3. 3

    University College London Hospitals NHS Foundation Trust, London, UK

  4. 4

    Haemoglobinopathy BMS Lead, Haematology Laboratory, St Mary's Hospital, Imperial College Healthcare NHS Trust, London, UK

Publication History

  1. Published Online: 18 NOV 2010
  2. Published Print: 28 NOV 2010

ISBN Information

Print ISBN: 9781405167154

Online ISBN: 9781444328318

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CHAPTER TOOLS

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Keywords:

  • Diagnostic procedures and principles of commonly used tests;
  • Clinical and family history;
  • Blood count and blood film;
  • High performance liquid chromatography;
  • Cellulose acetate membrane electrophoresis at alkaline pH;
  • Citrate agar gel electrophoresis at acid pH;
  • Agarose gel electrophoresis at acid pH;
  • Investigation of suspected thalassaemia;
  • Tests for instability of haemoglobin;
  • Tests for high and low affinity haemoglobins

Summary

This chapter contains sections titled:

  • Clinical and family history

  • Blood count and blood film

  • High performance liquid chromatography

  • Cellulose acetate membrane electrophoresis at alkaline pH

  • Citrate agar gel electrophoresis at acid pH

  • Agarose gel electrophoresis at acid pH

  • Isoelectric focusing

  • Capillary electrophoresis

  • Sickle solubility test

  • Haemoglobin H preparation

  • Tests for instability of haemoglobin

  • Tests for high and low affinity haemoglobins

  • Heinz body preparation

  • Kleihauer test

  • Tests for haemoglobin M

  • Investigation of suspected β thalassaemia

  • Investigation of suspected thalassaemia

  • Neonatal screening

  • DNA analysis

  • Mass spectrometry

  • References

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