10. Flow Cytometric Specimen Collection, Processing, and Reporting

  1. Kandice Kottke-Marchant MD, PhD4,5,6 and
  2. Bruce H. Davis MD7
  1. Maryalice Stetler-Stevenson MD, PhD1,
  2. Bruce Greig MT(ASCP)QCYM2 and
  3. Constance Yuan MD, PhD3

Published Online: 8 AUG 2012

DOI: 10.1002/9781444398595.ch10

Laboratory Hematology Practice

Laboratory Hematology Practice

How to Cite

Stetler-Stevenson, M., Greig, B. and Yuan, C. (2012) Flow Cytometric Specimen Collection, Processing, and Reporting, in Laboratory Hematology Practice (eds K. Kottke-Marchant and B. H. Davis), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444398595.ch10

Editor Information

  1. 4

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  2. 5

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  3. 6

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

  4. 7

    Trillium Diagnostics, LLC, Bangor, ME, USA

Author Information

  1. 1

    Flow Cytometry Unit, Laboratory of Pathology, CCR, NCI, NIH, Bethesda, MD, USA

  2. 2

    Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Vanderbilt University Medical Center, Nashville, TN, USA

  3. 3

    Laboratory of Pathology, NCI, NIH, Bethesda, MD, USA

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 10 APR 2012

ISBN Information

Print ISBN: 9781405162180

Online ISBN: 9781444398595



  • flow cytometry;
  • medical indications;
  • training;
  • method validation;
  • immunofluorescence staining;
  • interpretation of flow data;
  • reporting flow data


This chapter imparts detailed information on medical indications for flow cytometric testing, validation of flow cytometric methods, sample collection and processing, interpretation of flow cytometric data, the optimal format for reporting of diagnostic interpretations in the clinical setting, and requisite training for individuals in the field.