19. Engraftment Analysis Using Short Tandem Repeats Following Allogeneic Hematopoietic Cell Transplantation

  1. Kandice Kottke-Marchant MD, PhD3,4,5 and
  2. Bruce H. Davis MD6
  1. Ilka Warshawsky MD, PhD1 and
  2. Hyun-Sook Chi MD, PhD2

Published Online: 8 AUG 2012

DOI: 10.1002/9781444398595.ch19

Laboratory Hematology Practice

Laboratory Hematology Practice

How to Cite

Warshawsky, I. and Chi, H.-S. (2012) Engraftment Analysis Using Short Tandem Repeats Following Allogeneic Hematopoietic Cell Transplantation, in Laboratory Hematology Practice (eds K. Kottke-Marchant and B. H. Davis), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444398595.ch19

Editor Information

  1. 3

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  2. 4

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  3. 5

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

  4. 6

    Trillium Diagnostics, LLC, Bangor, ME, USA

Author Information

  1. 1

    Department of Molecular Pathology, Cleveland Clinic Foundation, Cleveland, OH, USA

  2. 2

    University of Ulsan, College of Medicine and Asan Medical Center, Seoul, Korea

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 10 APR 2012

ISBN Information

Print ISBN: 9781405162180

Online ISBN: 9781444398595

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Keywords:

  • chimerism;
  • short tandem repeats;
  • allogeneic transplantation;
  • polymerase chain reaction

Summary

Allogeneic hematopoietic cell transplantation is used to treat a variety of malignant and nonmalignant hematologic disorders. Chimerism analysis is an important tool for monitoring post-transplant outcome. Chimerism testing allows for documentation of successful engraftment and ablation of recipient cells. It also predicts untoward events including disease relapse, graft rejection, and graft-versus-host disease so that preemptive immunotherapy can be instituted and responses to treatment monitored. The most commonly used approach for chimerism testing is PCR amplification of microsatellites or short tandem repeat (STR) loci. This approach relies on differences between donor and recipient polymorphic genetic markers. This chapter focuses on STR-PCR, an informative and sensitive method to accurately quantitate amounts of donor and recipient DNA following allogeneic hematopoietic cell transplantation.