3. Automated Cell Analysis: Principles

  1. Kandice Kottke-Marchant MD, PhD2,3,4 and
  2. Bruce H. Davis MD5
  1. Bruce H. Davis MD5 and
  2. Patrick W. Barnes MA, MT(ASCP)1

Published Online: 8 AUG 2012

DOI: 10.1002/9781444398595.ch3

Laboratory Hematology Practice

Laboratory Hematology Practice

How to Cite

Davis, B. H. and Barnes, P. W. (2012) Automated Cell Analysis: Principles, in Laboratory Hematology Practice (eds K. Kottke-Marchant and B. H. Davis), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444398595.ch3

Editor Information

  1. 2

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  2. 3

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  3. 4

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

  4. 5

    Trillium Diagnostics, LLC, Bangor, ME, USA

Author Information

  1. 1

    Barnes-Jewish Hospital, St. Louis, MO, USA

  2. 5

    Trillium Diagnostics, LLC, Bangor, ME, USA

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 10 APR 2012

ISBN Information

Print ISBN: 9781405162180

Online ISBN: 9781444398595



  • blood cell count;
  • blood cell counter;
  • hematology instrument;
  • hematology analyzer;
  • blood count;
  • counting principles;
  • flow cytometer;
  • fluorescence;
  • red cell;
  • leukocytes;
  • platelets;
  • leukocyte differential


The automated complete blood cell count is performed using a number of different physical principles, such as electrical impedance, light scatter, and fluorescence. This chapter reviews the basic principles that form the basis of modern hematology blood cell counting instruments, as well as flow cytometry instruments.