32. Performance and Interpretation of Routine Coagulation Assays

  1. Kandice Kottke-Marchant MD, PhD1,2,3 and
  2. Bruce H. Davis MD4
  1. Kandice Kottke-Marchant MD, PhD1,2,3

Published Online: 8 AUG 2012

DOI: 10.1002/9781444398595.ch32

Laboratory Hematology Practice

Laboratory Hematology Practice

How to Cite

Kottke-Marchant, K. (2012) Performance and Interpretation of Routine Coagulation Assays, in Laboratory Hematology Practice (eds K. Kottke-Marchant and B. H. Davis), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444398595.ch32

Editor Information

  1. 1

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  2. 2

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  3. 3

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

  4. 4

    Trillium Diagnostics, LLC, Bangor, ME, USA

Author Information

  1. 1

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  2. 2

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  3. 3

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 10 APR 2012

ISBN Information

Print ISBN: 9781405162180

Online ISBN: 9781444398595

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Keywords:

  • hemostasis;
  • coagulation;
  • prothrombin time (PT/INR);
  • activated partial thromboplastin time (APTT);
  • fibrinogen;
  • reference ranges;
  • internal quality control;
  • external quality assessment

Summary

Hemostasis is a physiologic response to vascular injury involving the coagulation system, platelets, endothelial cells, and the vascular wall. Activation of the proteins of the coagulation system leads to the formation of thrombin and the conversion of fibrinogen into an insoluble fibrin clot. Abnormalities of the coagulation system can be detected by routine coagulation assays that include the prothrombin time (PT/INR), activated partial thromboplastin time (APTT), fibrinogen assay, and thrombin time. This chapter covers the laboratory performance of basic coagulation testing and discusses interpretation of testing results for diagnosis of bleeding disorders. The chapter also includes a discussion of assuring quality in the hemostasis laboratory through assay validation, setting appropriate reference ranges, internal quality control, and external quality assessment.