37. Platelet Assays and Platelet Dysfunction

  1. Kandice Kottke-Marchant MD, PhD2,3,4 and
  2. Bruce H. Davis MD5
  1. Paul Harrison BSc, PhD, FRCPath and
  2. David Keeling BSc, MD, FRCP, FRCPath

Published Online: 8 AUG 2012

DOI: 10.1002/9781444398595.ch37

Laboratory Hematology Practice

Laboratory Hematology Practice

How to Cite

Harrison, P. and Keeling, D. (2012) Platelet Assays and Platelet Dysfunction, in Laboratory Hematology Practice (eds K. Kottke-Marchant and B. H. Davis), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444398595.ch37

Editor Information

  1. 2

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  2. 3

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  3. 4

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

  4. 5

    Trillium Diagnostics, LLC, Bangor, ME, USA

Author Information

  1. Oxford Haemophilia & Thrombosis Centre, Churchill Hospital, Oxford, UK

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 10 APR 2012

ISBN Information

Print ISBN: 9781405162180

Online ISBN: 9781444398595



  • platelet;
  • platelet function;
  • platelet function testing;
  • platelet aggregation;
  • PFA-100;
  • bleeding time;
  • inherited platelet disorders;
  • acquired platelet disorders


Normal platelet function and number are critical for normal hemostasis and thus for rapid cessation of blood loss. This is illustrated by the range of both acquired and inherited qualitative and quantitative defects of platelets that can result in significant bleeding problems. A full clinical history and examination coupled with the application of first-line screening tests helps to define whether a patient may have an underlying platelet defect. Diagnosis is then usually confirmed by a variety of tests that include platelet aggregometry with various agonists, measurement of stored or released nucleotides, flow cytometry, and molecular biology. This chapter reviews the application of platelet function tests for the diagnosis of a wide range of platelet defects.