39. Lupus Anticoagulants, Antiphospholipid Antibodies, and Antiphospholipid Syndrome

  1. Kandice Kottke-Marchant MD, PhD9,10,11 and
  2. Bruce H. Davis MD12
  1. William L. Nichols MD1,2,
  2. Kandice Kottke-Marchant MD, PhD9,10,11,
  3. Marlies R. Ledford-Kraemer MBA, BS, MT(ASCP)SH3,
  4. Henry A. Homburger MD4,5,6 and
  5. Layna K. Cardel BS, MT(ASCP)7,8

Published Online: 8 AUG 2012

DOI: 10.1002/9781444398595.ch39

Laboratory Hematology Practice

Laboratory Hematology Practice

How to Cite

Nichols, W. L., Kottke-Marchant, K., Ledford-Kraemer, M. R., Homburger, H. A. and Cardel, L. K. (2012) Lupus Anticoagulants, Antiphospholipid Antibodies, and Antiphospholipid Syndrome, in Laboratory Hematology Practice (eds K. Kottke-Marchant and B. H. Davis), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444398595.ch39

Editor Information

  1. 9

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  2. 10

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  3. 11

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

  4. 12

    Trillium Diagnostics, LLC, Bangor, ME, USA

Author Information

  1. 1

    Divisions of Hematopathology and Hematology, Mayo Clinic Rochester, Rochester, MN, USA

  2. 2

    Medicine and Laboratory Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA

  3. 3

    CLOT - ED, Inc., NASCOLA, Islamorada, FL, USA

  4. 4

    Division of Clinical Biochemistry and Immunology, Mayo Clinic College of Medicine, Rochester, MN, USA

  5. 5

    Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, MN, USA

  6. 6

    Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, MN, USA

  7. 7

    Department of Laboratory Medicine and Pathology, Mayo Clinic Rochester, Rochester, MN, USA

  8. 8

    Laboratory Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA

  9. 9

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  10. 10

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  11. 11

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 10 APR 2012

ISBN Information

Print ISBN: 9781405162180

Online ISBN: 9781444398595

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Keywords:

  • anticardiolipin (aCL) antibodies;
  • antiphospholipid (aPL) antibodies;
  • antiphospholipid syndrome (APS);
  • β2-glycoprotein I (β2-GPI);
  • confirmatory assay;
  • lupus anticoagulant (LA);
  • mixing test;
  • thrombosis

Summary

The antiphospholipid syndrome (APS) is caused by heterogeneous auto-antibodies to various phospholipid-binding proteins, and manifests in an increased risk of arterial and venous thrombosis or recurrent pregnancy loss. Laboratory testing to support a diagnosis of APS encompasses phospholipid-dependent plasma clotting time tests for identification of the lupus anticoagulant (LA), together with serologic immunoassays for antibodies to β2-glycoprotein I (β2-GPI), with and/or without added cardiolipin (aCL) antibodies. The laboratory diagnosis of APS is complex and has been periodically refined with the publication of international guidelines. This chapter discusses approaches to the laboratory evaluation for LA and for antiphospholipid (aPL) antibodies.