48. Malaria Analysis

  1. Kandice Kottke-Marchant MD, PhD3,4,5 and
  2. Bruce H. Davis MD6
  1. Qigui Li MD, PhD1,
  2. Peter J. Weina PhD, MD, FACP, FIDSA2 and
  3. R. Scott Miller MD, FIDSA2

Published Online: 8 AUG 2012

DOI: 10.1002/9781444398595.ch48

Laboratory Hematology Practice

Laboratory Hematology Practice

How to Cite

Li, Q., Weina, P. J. and Miller, R. S. (2012) Malaria Analysis, in Laboratory Hematology Practice (eds K. Kottke-Marchant and B. H. Davis), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9781444398595.ch48

Editor Information

  1. 3

    Pathology & Laboratory Medicine Institute, Cleveland, OH, USA

  2. 4

    Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, OH, USA

  3. 5

    Hemostasis and Thrombosis, Department of Clinical Pathology, Cleveland Clinic, Cleveland, OH, USA

  4. 6

    Trillium Diagnostics, LLC, Bangor, ME, USA

Author Information

  1. 1

    Department of Pharmacology, Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Silver Spring, MD, USA

  2. 2

    Division of Experimental Therapeutics, Walter Reed Army Institute of Research, Silver Spring, MD, USA

Publication History

  1. Published Online: 8 AUG 2012
  2. Published Print: 10 APR 2012

ISBN Information

Print ISBN: 9781405162180

Online ISBN: 9781444398595

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Keywords:

  • malaria;
  • malaria analysis;
  • parasitemia;
  • microscopy;
  • antigen-based rapid diagnostic tools;
  • PCR;
  • flow cytometry;
  • resistance;
  • resistant markers;
  • antimalarials

Summary

The currently used malaria diagnostics provide some insight into possible new strategies for performing blood smear microscopy, antigen-based rapid diagnostic tests, PCR and drug-resistant assessments. Although many alternative diagnostic tests have been developed, the microscopy of Giemsa-stained thick and thin blood films is still the most widely used and universally accepted standard laboratory method. In the future though, the speed of tools such as antigen-based rapid diagnostic tools will provide an opportunity to extend the benefits of parasite-based diagnosis of malaria beyond the confines of light microscopy. Malaria analyses are also necessary and used in drug discovery programs for new antimalarials that use whole-organism, high-throughput screens. Surveillance for antimalarial resistance can be carried out either in vivo by measuring actual drug failure in patients or in vitro by detection of drug-resistant markers based on genetic changes associated with drug resistance that are likely confer to the parasite resistance to the drugs used to treat and prevent malaria. All analyses are reported here in detail.