Chapter 19. Site-Directed Spectroscopy and Site-Directed Chemistry of Biopolymers

  1. Deutsche Forschungsgemeinschaft (DFG)
  1. Stefan Limmer,
  2. Günther Ott and
  3. Mathias Sprinzl

Published Online: 21 DEC 2006

DOI: 10.1002/9783527609284.ch19

Macromolecular Systems: Microscopic Interactions and Macroscopic Properties: Final Report

Macromolecular Systems: Microscopic Interactions and Macroscopic Properties: Final Report

How to Cite

Limmer, S., Ott, G. and Sprinzl, M. (2000) Site-Directed Spectroscopy and Site-Directed Chemistry of Biopolymers, in Macromolecular Systems: Microscopic Interactions and Macroscopic Properties: Final Report (ed Deutsche Forschungsgemeinschaft (DFG)), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527609284.ch19

Author Information

  1. Biochemie, Universität Bayreuth, Universitätsstraße 30, 95447 Bayreuth, Germany

Publication History

  1. Published Online: 21 DEC 2006
  2. Published Print: 13 JUL 2000

ISBN Information

Print ISBN: 9783527277261

Online ISBN: 9783527609284

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Keywords:

  • macromolecular systems;
  • site-directed spectroscopy of biopolymers;
  • site-directed chemistry of biopolymers

Summary

This chapter contains sections titled:

  • Introduction

  • Site-specific NMR spectroscopy of chemically synthesized RNA duplexes

    • Stability of tRNA-derived acceptor stem duplexes

    • Manganese ion binding sites at RNA duplexes

    • Structural determination of short RNA duplexes by 2D NMR spectroscopy

    • NMR derived model of the tRNAAla acceptor arm

    • Chemical shifts and scalar coupling as an indicator of RNA structure in the vicinity of a G-U pair

    • Structure of aminoacyl-tRNA and transacylation of the aminoacyl residue

  • Structure of elongation factor Tu

    • Sequence of Thermus thermophilus EF-Tu

    • Crystallization, X-ray analysis, and the tertiary structure

    • Nucleotide binding and GTPase reaction

    • Mechanism of GTP induced conformational change of EF-Tu

    • Aminoacyl-tRNA in complex with EF-Tu ·GTP

    • 1H NMR of yeast Phe-tRNAPhe EF-Tu·GTP complex

    • 13C NMR studies of the Val-tRNAVal EF-Tu·GTP ternary complex

    • Role of EF-Tu in complex with aminoacyl-tRNA

    • EF-Tu interaction with EF-Ts

  • 3.10 Site-directed mutagenesis of EF-Tu

    • Summary and conclusions

    • Acknowledgement

    • References