Chapter 5. Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma

  1. Prof. Dr. Gilbert S. Omenn
  1. Lynn A. Echan,
  2. Hsin-Yao Tang,
  3. Nadeem Ali-Khan,
  4. KiBeom Lee and
  5. Dr. David W. Speicher

Published Online: 2 NOV 2006

DOI: 10.1002/9783527609482.ch5

Exploring the Human Plasma Proteome

Exploring the Human Plasma Proteome

How to Cite

Echan, L. A., Tang, H.-Y., Ali-Khan, N., Lee, K. and Speicher, D. W. (2006) Depletion of multiple high-abundance proteins improves protein profiling capacities of human serum and plasma, in Exploring the Human Plasma Proteome (ed G. S. Omenn), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527609482.ch5

Editor Information

  1. Internal Medicine, University of Michigan, A520 MSRB 1 Bldg, 1150 West Medical Center Dr. Ann Arbor, MI 48109–0626, USA

Author Information

  1. The Wistar Institute, 3601 Spruce St., Rm. 151, Philadelphia, PA 19104, USA

  1. Originally published in Proteomics 2005, 13, 3292–3303

Publication History

  1. Published Online: 2 NOV 2006
  2. Published Print: 6 OCT 2006

ISBN Information

Print ISBN: 9783527317578

Online ISBN: 9783527609482

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Keywords:

  • human plasma proteome;
  • depletion of multiple high-abundance proteins;
  • improves protein profiling capacities of human serum and plasma;
  • depletion of major proteins;
  • enhance detection of lower abundance proteins

Summary

This chapter contains sections titled:

  • Introduction

  • Materials and methods

    • Serum/plasma collection

    • MARS

    • Multiple affinity removal spin cartridge

    • Microscale solution IEF (MicroSol IEF) (ZOOMTM-IEF) fractionation

    • 2-DE

    • LC-MS/MS

  • Results

    • Depletion of major proteins to enhance detection of lower abundance proteins

    • Evaluation of high-abundance protein removal using 2-DE

    • Specificity of major protein depletion

    • Impact of Top-6 protein depletion on detection of lower abundance proteins using 2-D gels

    • Combining Top-6 protein depletion with microSol IEF prefractionation and narrow pH range gels

    • Analysis of Top-6 depleted serum and plasma using protein array pixelation

  • Discussion

  • References