Chapter 6. A novel four-dimensional strategy combining protein and peptide separation methods enables detection of low-abundance proteins in human plasma and serum proteomes

  1. Prof. Dr. Gilbert S. Omenn
  1. Hsin-Yao Tang,
  2. Nadeem Ali-Khan,
  3. Lynn A. Echan,
  4. Natasha Levenkova,
  5. John J. Rux and
  6. Dr. David W. Speicher

Published Online: 2 NOV 2006

DOI: 10.1002/9783527609482.ch6

Exploring the Human Plasma Proteome

Exploring the Human Plasma Proteome

How to Cite

Tang, H.-Y., Ali-Khan, N., Echan, L. A., Levenkova, N., Rux, J. J. and Speicher, D. W. (2006) A novel four-dimensional strategy combining protein and peptide separation methods enables detection of low-abundance proteins in human plasma and serum proteomes, in Exploring the Human Plasma Proteome (ed G. S. Omenn), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527609482.ch6

Editor Information

  1. Internal Medicine, University of Michigan, A520 MSRB 1 Bldg, 1150 West Medical Center Dr. Ann Arbor, MI 48109–0626, USA

Author Information

  1. The Wistar Institute, 3601 Spruce St., Rm. 151, Philadelphia, PA 19104, USA

  1. Originally published in Proteomics 2005, 13, 3329–3342

Publication History

  1. Published Online: 2 NOV 2006
  2. Published Print: 6 OCT 2006

ISBN Information

Print ISBN: 9783527317578

Online ISBN: 9783527609482

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Keywords:

  • human plasma proteome;
  • novel four-dimensional strategy combining protein and peptide separation methods;
  • detection of low-abundance proteins in human plasma and serum proteomes;
  • protein array pixelation strategy

Summary

This chapter contains sections titled:

  • Introduction

  • Materials and methods

    • Materials

    • Top six protein depletion

    • MicroSol-IEF fractionation

    • Protein array pixelation

    • LC-ESI-MS/MS methods

    • Data analysis

  • Results and discussion

    • Protein array pixelation strategy

    • Optimization of protein array pixelation

    • Total analysis time for protein array pixelation of human plasma proteome

    • Systematic protein array pixelation of the human plasma proteome

    • Systematic protein array pixelation of the human serum proteome

    • Analyses of human plasma and serum proteomes using HUPO filter criteria

  • Concluding remarks

  • References