Chapter 9. Efficient prefractionation of low-abundance proteins in human plasma and construction of a two-dimensional map

  1. Prof. Dr. Gilbert S. Omenn
  1. Sang Yun Cho,
  2. Eun-Young Lee,
  3. Joon Seok Lee,
  4. Hye-Young Kim,
  5. Jae Myun Park,
  6. Min-Seok Kwon,
  7. Young-Kew Park,
  8. Hyoung-Joo Lee,
  9. Min-Jung Kang,
  10. Jin Young Kim,
  11. Jong Shin Yoo,
  12. Sung Jin Park,
  13. Jin Won Cho,
  14. Hyon-Suk Kim and
  15. Professor Young-Ki Paik

Published Online: 2 NOV 2006

DOI: 10.1002/9783527609482.ch9

Exploring the Human Plasma Proteome

Exploring the Human Plasma Proteome

How to Cite

Cho, S. Y., Lee, E.-Y., Lee, J. S., Kim, H.-Y., Park, J. M., Kwon, M.-S., Park, Y.-K., Lee, H.-J., Kang, M.-J., Kim, J. Y., Yoo, J. S., Park, S. J., Cho, J. W., Kim, H.-S. and Paik, Y.-K. (2006) Efficient prefractionation of low-abundance proteins in human plasma and construction of a two-dimensional map, in Exploring the Human Plasma Proteome (ed G. S. Omenn), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527609482.ch9

Editor Information

  1. Internal Medicine, University of Michigan, A520 MSRB 1 Bldg, 1150 West Medical Center Dr. Ann Arbor, MI 48109–0626, USA

Author Information

  1. Yonsei Proteome Research Center and Biomedical Proteome Research Center, Yonsei University, 134 Shinchon-dong, Sudaemoon-ku, Seoul 120-749, Korea

  1. Originally published in Proteomics 2005, 13, 3386–3396

Publication History

  1. Published Online: 2 NOV 2006
  2. Published Print: 6 OCT 2006

ISBN Information

Print ISBN: 9783527317578

Online ISBN: 9783527609482

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Keywords:

  • human plasma proteome;
  • efficient prefractionation of low-abundance proteins;
  • construction of a two-dimensional map;
  • plasma sample preparation;
  • immunoaffinity column;
  • bioinformatics;
  • 2-DE map of human plasma devoid of high-abundance proteins

Summary

This chapter contains sections titled:

  • Introduction

  • Materials and methods

    • Plasma sample preparation

    • Depletion of major abundance proteins with an immunoaffinity column

    • 2-DE

    • Identification of proteins by MS

    • Fractionation of the plasma samples by FFE

    • LC-MS/MS

    • Bioinformatics

  • Results and discussion

    • 2-DE map of human plasma devoid of high-abundance proteins

    • Expression of different anticoagulant-treated plasma

    • FFE/1-DE/nanoLC-MS/MS and 2-DE/MALDI-TOF

  • Concluding remarks

  • References