Chapter 5. Methods for Solving the Phase Problem
Published Online: 5 JAN 2007
DOI: 10.1002/9783527610129.ch5
Copyright © 2007 Wiley-VCH Verlag GmbH & Co. KGaA
Book Title
X-Ray Crystallography of Biomacromolecules: A Practical Guide
Additional Information
How to Cite
Messerschmidt, A. (2007) Methods for Solving the Phase Problem, in X-Ray Crystallography of Biomacromolecules: A Practical Guide, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany. doi: 10.1002/9783527610129.ch5
Publication History
- Published Online: 5 JAN 2007
- Published Print: 15 DEC 2007
ISBN Information
Print ISBN: 9783527313969
Online ISBN: 9783527610129
- Summary
- Chapter
Keywords:
- methods for solving the phase problem;
- isomorphous replacement;
- anomalous scattering;
- determination of heavy-atom positions;
- phase calculations;
- Patterson search methods (molecular replacement);
- computer programs
Summary
This chapter contains sections titled:
Isomorphous Replacement
Preparation of Heavy-Metal Derivatives
Single Isomorphous Replacement
Multiple Isomorphous Replacement
Anomalous Scattering
Theoretical Background
Experimental Determination
Breakdown of Friedel's Law
Anomalous Difference Patterson Map
Phasing Including Anomalous Scattering Information
The Multiwavelength Anomalous Diffraction (MAD) Technique
Determination of the Absolute Configuration
Determination of Heavy-Atom Positions
Vector Verification Procedures
Direct Methods
Phase Calculation
Refinement of Heavy-Atom Parameters
Protein Phases
Maximum-Likelihood Parameter Refinement and Phase Calculation
Cross-Phasing of Heavy-Atom Derivatives or Anomalous Dispersion Data
Patterson Search Methods (Molecular Replacement)
Rotation Function
Locked Rotation Function
Translation Function
R-Factor and Correlation-Coefficient Translation Functions
Patterson-Correlation Translation Function
Phased Translation Function
Computer Programs for Molecular Replacement